Evidence map›Paper›PMID 36934637›Full record

ArticleTranslational oncology2023

Plasma exosome-derived circGAPVD1 as a potential diagnostic marker for colorectal cancer.

Tiankang Li, Tingting Zhou, Jin Wu, Heng Lv, Hui Zhou, Mingnan Du, Xiuzhong Zhang, Nai Wu, Shuai Gong, Zeqiang Ren and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational oncology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 21 citations in OpenAlex.

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  7. Role of Exosomal Non-Coding RNAsMicroRNA (Shariqah, United Arab Emirates) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Tiankang LiDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China; Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China.
Tingting ZhouDepartment of Endocrinology, The Affiliated Hospital of China University of Mining and Technology, Xuzhou, China; Department of Endocrinology, Xuzhou first People's Hospital, Xuzhou, China.
Jin WuDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China; Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China.
Heng LvDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China; Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China.
Hui ZhouDepartment of General Surgery, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Mingnan DuDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Xiuzhong ZhangDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Nai WuDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Shuai GongDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Zeqiang RenDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Pengbo ZhangDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Chong ZhangDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Guangpu LiuDepartment of Spinal Surgery, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou, China.
Xin LiuInstitute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China; Department of Endocrinology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. Electronic address: 489507198@qq.com.
Yi ZhangDepartment of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China; Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. Electronic address: dr_zhy@xzhmu.edu.cn.
Xuzhou Medical College · CNCentral South University · CNXuzhou No.1 People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAlthough circular RNAs (circRNAs) have recently garnered interest as disease markers, they have been relatively poorly studied as a biomarker in colorectal cancer (CRC). In this study, we aimed to screen the exosome-derived circRNAs in CRC and explore their potential as diagnostic and prognostic biomarkers of CRC

methodsExosomes were extracted from the plasma using a kit and validated by immunoblotting, transmission electron microscopy, and particle size analysis. The microarray datasets were employed to identify differentially-expressed circRNAs from plasma exosomes. Real-time quantitative reverse transcription PCR (RT-qPCR) verified the results of the microarray analysis, and Receiver operating characteristic (ROC) curve revealed the diagnostic ability of a single circRNA. The Starbase combined with microT, miRmap, and RNA22 were used to establish a circRNA-miRNA-mRNA network. Gene ontology, Kyoto Encyclopedia of Genes, Genomes pathway enrichment analysis, and Gene Set Enrichment Analysis were applied to determine potential functions of the identified mRNAs

resultsComparing the microarray of plasma exosome-derived circRNAs and the microarray downloaded from the GEO database, 15 candidate circRNAs with up-regulated expression were identified. RT-qPCR verified that hsa_circ_0003270 (circGAPVD1) was upregulated in CRC plasma exosomes. ROC analysis showed that circGAPVD1 in plasma exosomes has potential diagnostic value for CRC. The sensitivity and specificity of circGAPVD1 in the diagnosis of CRC were found to be 75.64 and 71.79%, respectively (area under ROC = 0.7662). Furthermore, the lymph node metastasis and TNM staging of patients were positively correlated with high expression of circGAPVD1. Combined with the ENCORI database and GEO datasets, we identified the circGAPVD1-related ceRNA network. The enrichment analysis revealed that key nodes in the ceRNA network participate in many important signaling pathways such as protein post-translational modifications

conclusionOur results revealed the diagnostic efficiency of circGAPVD1 in plasma exosomes. The highly expressed circGAPVD1 is expected to be a novel diagnostic marker for CRC.

Indexed as

BiomarkersceRNA networkCircular RNAColorectal cancerDiagnosisExosomes

Identifiers

PMID36934637
PMCPMC10034150
OpenAlexW4327756753

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.