Evidence map›Paper›PMID 36934281›Full record

SynthesisBMC oral health2023

Bacterial biofilm prevalence in dental unit waterlines: a systematic review and meta-analysis.

Mojtaba Bayani, Kiyavash Raisolvaezin, Amir Almasi-Hashiani, Seyed Hamed Mirhoseini

Full text readMeta-AnalysisSystematic Review
In one paragraph

Synthesis in BMC oral health, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Trial
  2. Article
  3. Article
  4. Long-term control ofFrontiers in oral health · 2026
    Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Veterinary Clinics as Reservoirs forAntibiotics (Basel, Switzerland) · 2025
    Review
  10. Pathogens (Basel, Switzerland) · 2025
    Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Dental Unit Waterlines: Disinfection and Management.International dental journal · 2024
    Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Tropical medicine and infectious disease · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mojtaba Bayani *Department of Periodontics, School of Dentistry, Arak University of Medical Sciences, Arak, Iran.
Kiyavash Raisolvaezin *Student Research Committee, Arak University of Medical Sciences, Arak, Iran.
Amir Almasi-HashianiDepartment of Epidemiology, School of Health, Arak University of Medical Sciences, Arak, Iran. Amiralmasi2007@gmail.com.
Seyed Hamed MirhoseiniDepartment of Environmental Health Engineering, School of Health, Arak University of Medical Sciences, Arak, Iran. hmirhossaini@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundsNumerous studies have shown that dental unit water lines (DUWLs) are often contaminated by a wide range of micro-organisms (bacteria, fungi, protozoa) and various prevalence have been reported for it in previous studies. Therefore, this review study aims to describe the prevalence of bacterial biofilm contamination of DUWLs.

methodsThis is a systematic review and meta-analysis in which the related keywords in different international databases, including Medline (via PubMed) and Scopus were searched. The retrieved studies were screened and the required data were extracted from the included studies. Three standard methods including American Dental Association (ADA), The Center for Disease Control and Prevention (CDC) and contaminated > 100 CFU/ml(C-100) standards were used to assess the bacterial biofilm contamination of DUWLs. All studies that calculated the prevalence of bacterial biofilm contamination of DUWLs, and English full-text studies were included in the meta-analysis. Studies that did not have relevant data or used unusual laboratory methods were excluded. Methodological risk of bias was assessed by a related checklist and finally, the data were pooled by fixed or random-effect models.

resultsSeven hundred and thirty-six studies were identified and screened and 26 related studies were included in the meta-analysis. The oldest included study was published in 1976 and the most recent study was published in 2020. According to the ADA, CDC and C-100 standards, the prevalence of bacterial contamination was estimated to be 85.0% (95% confidence interval (CI): 66.0-94.0%), 77.0% (95%CI: 66.0-85.0%) and 69.0% (95%CI: 67.0-71.0%), respectively. The prevalence of Legionella Pneumophila and Pseudomonas Aeruginosa in DUWLs was estimated to be 12.0% (95%CI: 10.0-14.0%) and 8.0% (95%CI: 2.0-24.0%), respectively.

conclusionThe results of this review study suggested a high prevalence of bacterial biofilm in DUWLs; therefore, the use of appropriate disinfecting protocol is recommended to reduce the prevalence of contamination and reduce the probable cross-infection.

Indexed as

BacteriaDental EquipmentBiofilmsColony Count, MicrobialEquipment ContaminationHumansPrevalenceBacterial BiofilmContaminationDental unitDental unit waterlineMeta-AnalysisMicrobial BiofilmMicrobial Contamination

Identifiers

PMID36934281
PMCPMC10024400

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.