Evidence map›Paper›PMID 36934147›Full record

ArticleScientific reports2023

Assessing compatibility and viral fitness between poultry-adapted H9N2 and wild bird-derived neuraminidases.

Anishia Wasberg, Inês R Faria, Julia Bergholm, Philipp P Petric, Ahmed Mostafa, Stephan Pleschka, Martin Schwemmle, Åke Lundkvist, Patrik Ellström, Mahmoud M Naguib

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 3 countries.

Anishia WasbergZoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Inês R FariaZoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Julia BergholmZoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Philipp P PetricInstitute of Virology, Medical Center, University of Freiburg, Freiburg, Germany.
Ahmed MostafaCenter of Scientific Excellence for Influenza Viruses, National Research Centre, Giza, Egypt.
Stephan PleschkaInstitute of Medical Virology, Justus Liebig University Giessen, Giessen, Germany.
Martin SchwemmleInstitute of Virology, Medical Center, University of Freiburg, Freiburg, Germany.
Åke LundkvistZoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Patrik EllströmZoonosis Science Center, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Mahmoud M NaguibZoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden. Mahmoud.naguib@imbim.uu.se.
Uppsala University · SEGerman Center for Infection Research · DENational Research Centre · EGUniversity Medical Center Freiburg · DEUniversity of Freiburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exchange of viral segments between one or more influenza virus subtypes can contribute to a shift in virulence and adaptation to new hosts. Among several influenza subtypes, H9N2 is widely circulating in poultry populations worldwide and has the ability to infect humans. Here, we studied the reassortant compatibility between chicken H9N2 with N1-N9 gene segments of wild bird origin, either with an intact or truncated stalk. Naturally occurring amino acid deletions in the NA stalk of the influenza virus can lead to increased virulence in both mallard ducks and chickens. Our findings show extended genetic compatibility between chicken H9Nx gene segments and the wild-bird NA with and without 20 amino acid stalk deletion. Replication kinetics in avian, mammalian and human cell lines revealed that parental chH9N2 and rH9N6 viruses with intact NA-stalk replicated significantly better in avian DF1 cells compared to human A549 cells. After introducing a stalk deletion, an enhanced preference for replication in mammalian and human cell lines could be observed for rH9N2

Indexed as

Influenza A Virus, H9N2 SubtypeInfluenza in BirdsAmino AcidsAnimalsAnimals, WildChickensHumansMammalsNeuraminidasePhylogenyPoultryAmino AcidsNeuraminidase

Identifiers

PMID36934147
PMCPMC10024770
OpenAlexW4327761291

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read22
table measurements read4
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.