ArticleThe Journal of cell biology1987
Visualization of cardiac ventricular myosin heavy chain homodimers and heterodimers by monoclonal antibody epitope mapping.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Alpha and beta myosin isoforms and human atrial and ventricular contraction.Cellular and molecular life sciences : CMLS · 2021Review
- Extracellular Superoxide Dismutase (EC-SOD) Regulates Gene Methylation and Cardiac Fibrosis During Chronic Hypoxic Stress.Frontiers in cardiovascular medicine · 2021Article
- Modeling smooth muscle myosin's two heads: long-lived enzymatic roles and phosphorylation-dependent equilibria.Biophysical journal · 2010Article
- A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles.Plant physiology · 2009Article
- Single-headed myosin II acts as a dominant negative mutation in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Expression of myosin heavy and light chains and phosphorylation of the phosphorylatable myosin light chain in the heart ventricle of the European hamster during hibernation and in summer.Journal of muscle research and cell motility · 1992Article
- Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development.The Journal of cell biology · 1991Article
- Assembly of avian skeletal muscle myosins: evidence that homodimers of the heavy chain subunit are the thermodynamically stable form.The Journal of cell biology · 1991Article
- Isolated dystrophin molecules as seen by electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Expression of human beta-myosin heavy chain fragments in Escherichia coli; localization of actin interfaces on cardiac myosin.Journal of muscle research and cell motility · 1990Article
- The distribution of heavy-chain isoforms of myosin in airways smooth muscle from adult and neonate humans.The Biochemical journal · 1989Article
- Immunogenetic basis of myocarditis: role of fibrillary antigens.Springer seminars in immunopathology · 1989Review
- Distribution pattern of alpha and beta myosin in normal and diseased human ventricular myocardium.Basic research in cardiologyArticle
- Different ratio of myosin heavy chain isoforms in arterial smooth muscle of spontaneously hypertensive rats.Basic research in cardiologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two mAbs, one specific for cardiac alpha-myosin heavy chains (MHC) and the other specific for cardiac beta-MHC, were used to investigate the heavy-chain dimeric organization of rat cardiac ventricular myosin. Epitopes of the two mAbs were mapped on the myosin molecule by electron microscopy of rotary shadowed mAb-myosin complexes. mAbs were clearly identifiable by the different locations of their binding sites on the myosin rod. Thus, myosin molecules could be directly discriminated according to their alpha-or beta-MHC content. alpha alpha-MHC and beta beta-MHC homodimers were visualized in complexes consisting of two molecules of the same mAb bound to one myosin molecule. By simultaneously using the alpha-MHC-specific mAb and the beta-MHC-specific mAb, alpha beta-MHC heterodimers were visualized in complexes formed by one molecule of each of the two mAbs bound to one myosin molecule. Proportions of alpha alpha-and beta beta-MHC homodimers and alpha beta-MHC heterodimers were estimated from quantifications of mAb-myosin complexes and compared with the proportions given by electrophoreses under nondenaturing conditions. This visualization of cardiac myosin molecules clearly demonstrates the arrangement of alpha- and beta-MHC in alpha alpha-MHC homodimers, beta beta-MHC homodimers, and alpha beta-MHC heterodimers, as initially proposed by Hoh, J. F. Y., G. P. S. Yeoh, M. A. W. Thomas, and L. Higginbottom (1979).
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