ArticleThe Journal of cell biology1987
Cytosolic free calcium increases before and oscillates during frustrated phagocytosis in macrophages.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Localisation of Intracellular Signals and Responses during Phagocytosis.International journal of molecular sciences · 2023Review
- Efferocytosis requires periphagosomal CaNature communications · 2022Article
- Quiescence of Human Monocytes after Affinity Purification: A Novel Method Apt for Monocyte Stimulation Assays.Biomolecules · 2022Article
- β-1,3/1,6-Glucans and Immunity: State of the Art and Future Directions.Molecular nutrition & food research · 2021Review
- Review
- Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading.Scientific reports · 2017Article
- The role of STIM and ORAI proteins in phagocytic immune cells.American journal of physiology. Cell physiology · 2016Review
- Opposing Effects of NGF and proNGF on HIV Induced Macrophage Activation.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2016Article
- TRPC6 channel translocation into phagosomal membrane augments phagosomal function.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Differential regulation of macrophage phenotype by mature and pro-nerve growth factor.Journal of neuroimmunology · 2015Article
- Ca²⁺ and calpain control membrane expansion during the rapid cell spreading of neutrophils.Journal of cell science · 2013Article
- Connexin-43 hemichannels mediate cyclic ADP-ribose generation and its Ca2+-mobilizing activity by NAD+/cyclic ADP-ribose transport.The Journal of biological chemistry · 2011Article
- RhoA regulates calcium-independent periodic contractions of the cell cortex.Biophysical journal · 2010Article
- Frequent calcium oscillations lead to NFAT activation in human immature dendritic cells.The Journal of biological chemistry · 2010Article
- Ion channel clustering enhances weak electric field detection by neutrophils: apparent roles of SKF96365-sensitive cation channels and myeloperoxidase trafficking in cellular responses.European biophysics journal : EBJ · 2005Article
- Cellular memory: neutrophil orientation reverses during temporally decreasing chemoattractant concentrations.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Oscillatory pericellular proteolysis and oxidant deposition during neutrophil locomotion.Biophysical journal · 1998Article
- Proximity oscillations of complement type 4 (alphaX beta2) and urokinase receptors on migrating neutrophils.Biophysical journal · 1997Article
- Human macrophages contain a stretch-sensitive potassium channel that is activated by adherence and cytokines.The Journal of membrane biology · 1995Article
- Activation of a potassium outward current by zymosan and opsonized zymosan in mouse peritoneal macrophages.Naunyn-Schmiedeberg's archives of pharmacology · 1994Article
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2 authors.
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Abstract
When macrophages and neutrophils are allowed to settle onto an appropriate surface, they attach and spread in a frustrated attempt to phagocytose the substrate. Spreading is associated with extensive rearrangements of the actin cytoskeleton which resemble those occurring during phagocytosis. We have previously shown that spreading in human neutrophils is preceded by an increase in cytosolic-free calcium concentration [( Ca2+]i) (Kruskal, B. A., S. Shak, and F. R. Maxfield. 1986. Proc. Natl. Acad. Sci. USA. 83:2919-2923). To assess the generality of this signal, we measured [Ca2+]i in single thioglycollate-elicited mouse peritoneal macrophages as they spread on an immune complex-coated surface, using fura-2 microspectrofluorometry. A [Ca2+]i increase always precedes spreading. This increase can involve several (up to 8) [Ca2+]i spikes, with an average peak value of 387 +/- 227 nM (mean +/- SD, n = 92 peaks in 24 cells), before spreading is detected. Neither spreading nor the magnitude of these spikes is significantly altered by removal of extracellular calcium. Many of the spreading macrophages exhibit periodic [Ca2+]i increases before and during spreading. The proportion which does so varies among experiments from 0 to 90%, but it is frequently greater than 40%. The largest number of cells (approximately 25%) exhibited only a single peak. In 13 cells that showed more than 10 peaks, the median period was 29 s (range 19-69 s). The average peak [Ca2+]i was 385 +/- 266 nM (mean +/- SD, n = 208 peaks in 14 cells). The calcium producing these increases is derived from intracellular pools. The oscillations occur with spreading on either opsonized or nonopsonized surfaces. The function of these oscillations is not clear, but the large number of cells which exhibit them suggest that they may be important to macrophage function.
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