ArticleNature communications2023
Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- An orthogonal transcription system enables high-efficiency rescue of measles virus-based vaccine vectors against multiple pathogens.Synthetic and systems biotechnology · 2027Article
- A decoupled transcription platform enables tunable and predictable gene expression in yeast.Nature communications · 2026Article
- Mammalian synthetic gene circuits for biopharmaceutical development & manufacture.NPJ systems biology and applications · 2025Review
- Engineered Bacteria-Nano Hybrid System: The Intelligent Drug Factory for Next-Generation Cancer Immunotherapy.Pharmaceutics · 2025Review
- Development Strategies for Influenza Vaccines Utilizing Phage RNA Polymerase and Capping Enzyme NP868R.Chem & bio engineering · 2025Article
- Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae.Nature communications · 2025Article
- De novo design of insulated cis-regulatory elements based on deep learning-predicted fitness landscape.Nucleic acids research · 2025Article
- Discovery of diverse and high-quality mRNA capping enzymes through a language model-enabled platform.Science advances · 2025Article
- Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes.iScience · 2025Article
- Predictive genetic circuit design for phenotype reprogramming in plants.Nature communications · 2025Article
- Bio-encapsulation of allergen-derivatives for specific immunotherapy.Frontiers in plant science · 2025Article
- Engineering signalling pathways in mammalian cells.Nature biomedical engineering · 2024Review
- Synthetic translational coupling element for multiplexed signal processing and cellular control.Nucleic acids research · 2024Article
- Directed evolution of an orthogonal transcription engine for programmable gene expression in eukaryotes.bioRxiv : the preprint server for biology · 2024Article
- SEMPER: Stoichiometric expression of mRNA polycistrons by eukaryotic ribosomes for compact, ratio-tunable multi-gene expression.Cell systems · 2024Article
- Context-dependent redesign of robust synthetic gene circuits.Trends in biotechnology · 2024Review
- Resource-aware construct design in mammalian cells.Nature communications · 2023Article
- Resource allocation in mammalian systems.Biotechnology advancesReview
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Authors and funding
13 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Context-dependency of mammalian transcriptional elements has hindered the quantitative investigation of multigene expression stoichiometry and its biological functions. Here, we describe a host- and local DNA context-independent transcription system to gradually fine-tune single and multiple gene expression with predictable stoichiometries. The mammalian transcription system is composed of a library of modular and programmable promoters from bacteriophage and its cognate RNA polymerase (RNAP) fused to a capping enzyme. The relative expression of single genes is quantitatively determined by the relative binding affinity of the RNAP to the promoters, while multigene expression stoichiometry is predicted by a simple biochemical model with resource competition. We use these programmable and modular promoters to predictably tune the expression of three components of an influenza A virus-like particle (VLP). Optimized stoichiometry leads to a 2-fold yield of intact VLP complexes. The host-independent orthogonal transcription system provides a platform for dose-dependent control of multiple protein expression which may be applied for advanced vaccine engineering, cell-fate programming and other therapeutic applications.
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