Evidence map›Paper›PMID 36930747›Full record

ArticleAdvanced healthcare materials2023

Mesenchymal Stem Cell Culture within Perfusion Bioreactors Incorporating 3D-Printed Scaffolds Enables Improved Extracellular Vesicle Yield with Preserved Bioactivity.

Stephanie M Kronstadt, Divya B Patel, Louis J Born, Daniel Levy, Max J Lerman, Bhushan Mahadik, Shannon T McLoughlin, Arafat Fasuyi, Lauren Fowlkes, Lauren Hoorens Van Heyningen and 8 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed, 2 pooled it
8.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 2 syntheses or guidelines pooled it, 54 citations in OpenAlex.

  1. Pooled it
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  15. 80 years of extracellular vesicles: from discovery to clinical translation.Extracellular vesicles and circulating nucleic acids · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 2 institutions in 1 country.

Stephanie M KronstadtFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Divya B PatelFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Louis J BornFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Daniel LevyFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Max J LermanFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Bhushan MahadikFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Shannon T McLoughlinFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Arafat FasuyiFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Lauren FowlkesFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Lauren Hoorens Van HeyningenFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Amaya ArandaFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Sanaz Nourmohammadi AbadchiHendrix Burn and Wound Healing Laboratory, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, 21224, USA.
Kai-Hua ChangHendrix Burn and Wound Healing Laboratory, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, 21224, USA.
Angela Ting Wei HsuHendrix Burn and Wound Healing Laboratory, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, 21224, USA.
Sameer BengaliHendrix Burn and Wound Healing Laboratory, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, 21224, USA.
John W HarmonHendrix Burn and Wound Healing Laboratory, Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, 21224, USA.
John P FisherFischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
Steven M JayProgram in Molecular and Cell Biology, University of Maryland, College Park, MD, 20742, USA.ORCID 0000-0002-3827-5988
University of Maryland, College Park · USJohns Hopkins University · US

Funding

Training and Dissemination CoreP41EB023833 · NIBIB · UNIV OF MARYLAND, COLLEGE PARK · PI FISHER, JOHN P, MIKOS, ANTONIOS G. · 2017 to 2021
$6.2M
Characterization of the Cardiac Progenitor Cell Exosomes for Optimal TherapeuticsR01HL141922 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI KAUSHAL, SUNJAY · 2019 to 2022
$2.8M
Dementia Following Spinal Cord Injury: Mechanism and Therapeutic TargetingRF1NS110637 · NINDS · UNIVERSITY OF MARYLAND BALTIMORE · PI JAY, STEVEN MICHAEL, WU, JUNFANG · 2019 to 2019
$2.8M
Controlling Exosome Noncoding RNA Cargo for Enhanced Wound HealingR01HL141611 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI JAY, STEVEN MICHAEL · 2018 to 2022
$1.9M
Enabling exosome biomarker development via digitized single vesicle analysisR01GM130923 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI DEVOE, DON L, WHITE, IAN M · 2019 to 2022
$1.7M
Nanohydrocyclones for scalable extracellular vesicle purification and drug loadingR21HL159590 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI DEVOE, DON L, JAY, STEVEN MICHAEL · 2021 to 2022
$421k
NHLBI NIH HHS R01 HL141611NHLBI NIH HHS R01 HL141922NHLBI NIH HHS R21 HL159590NIBIB NIH HHS P41 EB023833NIGMS NIH HHS R01 GM130923NINDS NIH HHS RF1 NS110637
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are implicated as promising therapeutics and drug delivery vehicles in various diseases. However, successful clinical translation will depend on the development of scalable biomanufacturing approaches, especially due to the documented low levels of intrinsic EV-associated cargo that may necessitate repeated doses to achieve clinical benefit in certain applications. Thus, here the effects of a 3D-printed scaffold-perfusion bioreactor system are assessed on the production and bioactivity of EVs secreted from bone marrow-derived mesenchymal stem cells (MSCs), a cell type widely implicated in generating EVs with therapeutic potential. The results indicate that perfusion bioreactor culture induces an ≈40-80-fold increase (depending on measurement method) in MSC EV production compared to conventional cell culture. Additionally, MSC EVs generated using the perfusion bioreactor system significantly improve wound healing in a diabetic mouse model, with increased CD31

Indexed as

Extracellular VesiclesMesenchymal Stem CellsAnimalsBioreactorsMicePerfusionPrinting, Three-Dimensional3D printingbioreactorsexosomemesenchymal stem cellswound healing

Identifiers

PMID36930747
PMCPMC10505252
OpenAlexW4327709736

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.