ArticlePlant physiology2023
Riboflavin mediates m6A modification targeted by miR408, promoting early somatic embryogenesis in longan.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Synergistic Promotion of Somatic Embryogenesis inPlants (Basel, Switzerland) · 2026Article
- Auxin-induced AsARF16 complex orchestrates lncRNA125175-mediated ceRNA networks to regulate garlic somatic embryogenesis.Horticulture research · 2026Article
- The eTM-miR3699-MAN7 mediated cell wall degradation in regulating embryogenic cell formation during the early stage of somatic embryogenesis in apple.Horticulture research · 2026Article
- Adsorption of Per- and Polyfluoroalkyl Substances by Edible Nutraceutical-Amended Montmorillonite Clays: In Vitro, In Vivo and In Silico Enterosorption Strategies.Water, air, and soil pollution · 2025Article
- Functional and Transcriptome Analysis Reveal Specific Roles ofPlants (Basel, Switzerland) · 2024Article
- Detection, distribution, and functions of RNAFrontiers in plant science · 2024Review
- Genome-Wide Identification and Expression Analysis Reveals the B3 Superfamily Involved in Embryogenesis and Hormone Responses inInternational journal of molecular sciences · 2023Article
- Epigenetic modifications and miRNAs determine the transition of somatic cells into somatic embryos.Plant cell reports · 2023Review
- Uncovering the miRNA-mediated regulatory network involved in Ma bamboo (Horticulture research · 2023Article
- Focus on fruit crops.Plant physiology · 2023Article
- Transcription factors NF-YB involved in embryogenesis and hormones responses inFrontiers in plant science · 2023Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant somatic embryogenesis (SE) is an in vitro biological process wherein bipolar structures are induced to form somatic cells and regenerate into whole plants. MicroRNA (miRNA) is an essential player in plant SE. However, the mechanism of microRNA408 (miR408) in SE remains elusive. Here, we used stable transgenic technology in longan (Dimocarpus longan) embryogenic calli to verify the mechanism by which miR408 promotes cell division and differentiation of longan early SE. dlo-miR408-3p regulated riboflavin biosynthesis by targeting nudix hydrolase 23 (DlNUDT23), a previously unidentified gene mediating N6-methyladenosine (m6A) modification and influencing RNA homeostasis and cell cycle gene expression during longan early SE. We showed that DlMIR408 overexpression (DlMIR408-OE) promoted 21-nt miRNA biosynthesis. In DlMIR408-OE cell lines, dlo-miR408-3p targeted and downregulated DlNUDT23, promoted riboflavin biosynthesis, decreased flavin mononucleotide (FMN) accumulation, promoted m6A level, and influenced miRNA homeostasis. DNA replication, glycosylphosphatidylinositol (GPI)-anchor biosynthesis, the pentose phosphate pathway, and taurine and hypotaurine metabolism were also closely associated with riboflavin metabolism. In a riboflavin feeding assay, dlo-miR408-3p and pre-miR408 were upregulated and DlNUDT23 was downregulated, increasing the m6A level and cell division and differentiation in longan globular embryos. When riboflavin biosynthesis was inhibited, dlo-miR408-3p was downregulated and DlNUDT23 was upregulated, which decreased m6A modification and inhibited cell division but did not inhibit cell differentiation. FMN artificial demethylated m6A modification affected the homeostasis of precursor miRNA and miRNA. Our results revealed a mechanism underlying dlo-miR408-3p-activated riboflavin biosynthesis in which DlNUDT23 is targeted, m6A modification is dynamically mediated, and cell division is affected, promoting early SE in plants.
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