ReviewMethods in molecular biology (Clifton, N.J.)2023
Statistical Analysis of Multiplex Immunofluorescence and Immunohistochemistry Imaging Data.
Review in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Topology-Based Biomarkers Accurately Predict Breast Cancer Outcome and Survival.Cancer research · 2026Article
- Topological Data Analysis of Spatial Protein Expression in Multiplexed Spatial Proteomics Studies.bioRxiv : the preprint server for biology · 2026Article
- Detecting clinically relevant topological structures in multiplexed spatial proteomics using TopKAT.Patterns (New York, N.Y.) · 2026Article
- Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining.Histology and histopathology · 2025Article
- Quantile index predictors using R package hyper.gam.Bioinformatics (Oxford, England) · 2025Article
- Functional protein biomarkers based on distributions of expression levels in single-cell imaging data.Bioinformatics (Oxford, England) · 2025Article
- The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells.Discover oncology · 2025Article
- Benchmarking Spatial Co-Localization Methods for Single-Cell Multiplex Imaging Data with Applications to High-Grade Serous Ovarian and Triple Negative Breast Cancer.Statistics and data science in imaging · 2025Article
- Statistical analysis of multiple regions-of-interest in multiplexed spatial proteomics data.Briefings in bioinformatics · 2024Article
- A Spatial Omnibus Test (SPOT) for Spatial Proteomic Data.Bioinformatics (Oxford, England) · 2024Article
- SpaceANOVA: Spatial Co-occurrence Analysis of Cell Types in Multiplex Imaging Data Using Point Process and Functional ANOVA.Journal of proteome research · 2024Article
- A Spatial Omnibus Test (SPOT) for Spatial Proteomic Data.bioRxiv : the preprint server for biology · 2024Article
- mxfda: a comprehensive toolkit for functional data analysis of single-cell spatial data.Bioinformatics advances · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Advances in multiplexed single-cell immunofluorescence (mIF) and multiplex immunohistochemistry (mIHC) imaging technologies have enabled the analysis of cell-to-cell spatial relationships that promise to revolutionize our understanding of tissue-based diseases and autoimmune disorders. Multiplex images are collected as multichannel TIFF files; then denoised, segmented to identify cells and nuclei, normalized across slides with protein markers to correct for batch effects, and phenotyped; and then tissue composition and spatial context at the cellular level are analyzed. This chapter discusses methods and software infrastructure for image processing and statistical analysis of mIF/mIHC data.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.