ArticleNature plants2023
Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 57 citations in OpenAlex.
- Subspecies-specific metabolic gene clusters shape polyphenol dynamics for modular metabolic breeding in sugarcane.Plant communications · 2026Article
- Novel effector HYPB1 of cotton bollworm (Helicoverpa armigera) inhibits biosynthesis of plant secondary metabolites and promotes feeding by targeting cotton dirigent protein GhDIR15.Journal of integrative plant biology · 2026Article
- Biosynthetic mechanism and ecological function of chirality in Solanum steroidal alkaloids.Nature communications · 2026Article
- Review
- Three Decades of China's Bt Cotton: Achievements and Insights.Plant biotechnology journal · 2026Review
- A pangenome reference and population studies link structural variants with breeding traits in Gossypium hirsutum.Nature genetics · 2026Article
- Microbial Mediators of Pine Defense Resistance: Stage-Specific Gut Symbionts Enable Acantholyda posticalis to Overcome Terpenoid Barriers.Microbial ecology · 2026Article
- A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges.Nature communications · 2025Article
- CRISPR/Cas9-mediated mutation of GhCAD decreases the gossypol content of cottonseed.Journal of biological engineering · 2025Article
- Effects of Gossypol Exposure on Ovarian Reserve Function: Comprehensive Risk Assessment Based on TRAEC Strategy.Toxics · 2025Article
- Article
- AaMYC3 bridges the regulation of glandular trichome density and artemisinin biosynthesis in Artemisia annua.Plant biotechnology journal · 2025Article
- Improved quality of cottonseed meal: effect of cottonseed protein isolate on growth performance, nutrient digestibility, and intestinal health in growing pigs.Journal of animal science · 2025Article
- Advances in genomic tools for plant breeding: harnessing DNA molecular markers, genomic selection, and genome editing.Biological research · 2024Review
- The Potential of CRISPR/Cas9 to Circumvent the Risk Factor NeurotoxinCurrent issues in molecular biology · 2024Review
- Regulation of Glandular Size and Phytoalexin Biosynthesis by a Negative Feedback Loop in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The ERF transcription factor LTF1 activates DIR1 to control stereoselective synthesis of antiviral lignans and stress defense inActa pharmaceutica Sinica. B · 2024Article
- The resilient cotton plant: uncovering the effects of stresses on secondary metabolomics and its underlying molecular mechanisms.Functional & integrative genomics · 2023Review
- Genome-wide identification and expression profiling analysis ofFrontiers in plant science · 2023Article
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14 authors at 6 institutions in 2 countries.
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Abstract
Axial chirality of biaryls can generate varied bioactivities. Gossypol is a binaphthyl compound made by cotton plants. Of its two axially chiral isomers, (-)-gossypol is the bioactive form in mammals and has antispermatogenic activity, and its accumulation in cotton seeds poses health concerns. Here we identified two extracellular dirigent proteins (DIRs) from Gossypium hirsutum, GhDIR5 and GhDIR6, which impart the hemigossypol oxidative coupling into (-)- and (+)-gossypol, respectively. To reduce cotton seed toxicity, we disrupted GhDIR5 by genome editing, which eliminated (-)-gossypol but had no effects on other phytoalexins, including (+)-gossypol, that provide pest resistance. Reciprocal mutagenesis identified three residues responsible for enantioselectivity. The (-)-gossypol-forming DIRs emerged later than their enantiocomplementary counterparts, from tandem gene duplications that occurred shortly after the cotton genus diverged. Our study offers insight into how plants control enantiomeric ratios and how to selectively modify the chemical spectra of cotton plants and thereby improve crop quality.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.