ArticleNature plants2023
Synthetic maize centromeres transmit chromosomes across generations.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Technologies for engineering repetitive DNA.Quantitative biology (Beijing, China) · 2026Review
- T2T Genomes Unveil Centromere Architecture and Adaptive Divergence in Large Yellow Croaker (Larimichthys crocea).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- RepeatOBserver: Tandem Repeat Visualisation and Putative Centromere Detection.Molecular ecology resources · 2025Article
- Increased maize chromosome number by engineered chromosome fission.Science advances · 2025Article
- Integrating Arabidopsis and crop species gene discovery for crop improvement.The Plant cell · 2025Review
- Four near-complete genome assemblies reveal the landscape and evolution of centromeres in Salicaceae.Genome biology · 2025Article
- The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025Review
- Past innovations and future possibilities in plant chromosome engineering.Plant biotechnology journal · 2025Review
- Centromere diversity and its evolutionary impacts on plant karyotypes and plant reproduction.The New phytologist · 2025Review
- Can a plant biologist fix a thermostat?The New phytologist · 2025Review
- Pioneers of chromosome elimination.Frontiers in epigenetics and epigenomics · 2025Article
- Bridging the gap: unravelling plant centromeres in the telomere-to-telomere era.The New phytologist · 2024Review
- Unveiling the distinctive traits of functional rye centromeres: minisatellites, retrotransposons, and R-loop formation.Science China. Life sciences · 2024Article
- Synthetic moss.Nature plants · 2024Article
- Plant reproduction research in Latin America: Toward sustainable agriculture in a changing environment.Plant-environment interactions (Hoboken, N.J.) · 2024Review
- The structure, function, and evolution of plant centromeres.Genome research · 2024Review
- A designer synthetic chromosome fragment functions in moss.Nature plants · 2024Article
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Towards Plant Synthetic Genomics.Biodesign research · 2023Article
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Abstract
Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a tethering approach that activates functional maize centromeres at synthetic sequence arrays. A LexA-CENH3 fusion protein was used to recruit native Centromeric Histone H3 (CENH3) to long arrays of LexO repeats on a chromosome arm. Newly recruited CENH3 was sufficient to organize functional kinetochores that caused chromosome breakage, releasing chromosome fragments that were passed through meiosis and into progeny. Several fragments formed independent neochromosomes with centromeres localized over the LexO repeat arrays. The new centromeres were self-sustaining and transmitted neochromosomes to subsequent generations in the absence of the LexA-CENH3 activator. Our results demonstrate the feasibility of using synthetic centromeres for karyotype engineering applications.
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