Evidence map›Paper›PMID 36927857›Full record

ArticleThe Journal of veterinary medical science2023

Analysis of gene expression in poultry red mite, Dermanyssus gallinae, by RNAscope in situ hybridization.

Hikari Seo, Shiro Murata, Osamu Ichii, Takashi Namba, Shwe Yee Win, Takumi Sato, Eiji Oishi, Akira Taneno, Naoya Maekawa, Tomohiro Okagawa and 2 more

Open access · diamondAbstract read
In one paragraph

Article in The Journal of veterinary medical science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Hikari SeoDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Shiro MurataDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Osamu IchiiDepartment of Basic Veterinary Science, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Takashi NambaDepartment of Basic Veterinary Science, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Shwe Yee WinDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Takumi SatoVaxxinova Japan K.K., Tokyo, Japan.
Eiji OishiVaxxinova Japan K.K., Tokyo, Japan.
Akira TanenoVaxxinova Japan K.K., Tokyo, Japan.
Naoya MaekawaDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Tomohiro OkagawaDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Satoru KonnaiDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Kazuhiko OhashiDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Hokkaido University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The poultry red mite (PRM; Dermanyssus gallinae) is a hematophagous ectoparasite that mainly infests chickens, and its infestation causes significant economic losses to the poultry industry. In this study, we examined the use of RNAscope-based in situ hybridization (ISH) to characterize gene expression in PRM. We analyzed the mRNA expression of Dermanyssus gallinae cathepsin D-1 (Dg-CatD-1) and Dermanyssus gallinae cystatin (Dg-Cys). RNAscope ISH analysis revealed that mRNA expression of Dg-CatD-1 was observed in the digestive tract, and Dg-Cystatin mRNA was expressed in the ovaries in addition to the digestive tract. RNAscope ISH could be applicable for the analysis of gene expression in each tissue of PRM and is an effective method to investigate the characteristics of target genes.

Indexed as

Mite InfestationsMitesPoultry DiseasesAnimalsChickensGene ExpressionPoultrycathepsin D-1cystatinin situ hybridizationpoultry red mitesRNAscope

Identifiers

PMID36927857
PMCPMC10209471
OpenAlexW4324117657

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.