ArticleAnalytical chemistry2023
Determination of Intracellular Esterase Activity Using Ratiometric Raman Sensing and Spectral Phasor Analysis.
Article in Analytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Sensitive Detection and Identification Method of Erythrocyte-like Cells upon Doxorubicin Induced Differentiation with Vibrational Techniques.Analytical chemistry · 2025Article
- Remote Silyl Groups Enhance Hydrolytic Stability and Photocleavage Efficiency in Carbamates for Protein Release.Angewandte Chemie (International ed. in English) · 2025Article
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Principle of Stimulated Raman Scattering Microscopy: Emerging at High Spatiotemporal Limits.The journal of physical chemistry. C, Nanomaterials and interfaces · 2025Article
- Abridgement of Microbial Esterases and Their Eminent Industrial Endeavors.Molecular biotechnology · 2025Review
- Label-Free Screening of Drug-Induced Liver Injury Using Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis.Analytical chemistry · 2024Article
- Programmable hyperspectral coherent anti-Stokes Raman scattering microscopy.Optics letters · 2024Article
- Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level.ACS central science · 2024Review
- Biomedical applications, perspectives and tag design concepts in the cell - silent Raman window.RSC chemical biology · 2024Review
- Expanding the Range of Bioorthogonal Tags for Multiplex Stimulated Raman Scattering Microscopy.Angewandte Chemie (International ed. in English) · 2023Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carboxylesterases (CEs) are a class of enzymes that catalyze the hydrolysis of esters in a variety of endogenous and exogenous molecules. CEs play an important role in drug metabolism, in the onset and progression of disease, and can be harnessed for prodrug activation strategies. As such, the regulation of CEs is an important clinical and pharmaceutical consideration. Here, we report the first ratiometric sensor for CE activity using Raman spectroscopy based on a bisarylbutadiyne scaffold. The sensor was shown to be highly sensitive and specific for CE detection and had low cellular cytotoxicity. In hepatocyte cells, the ratiometric detection of esterase activity was possible, and the result was validated by multimodal imaging with standard viability stains used for fluorescence microscopy within the same cell population. In addition, we show that the detection of localized ultraviolet damage in a mixed cell population was possible using stimulated Raman scattering microscopy coupled with spectral phasor analysis. This sensor demonstrates the practical advantages of low molecular weight sensors that are detected using ratiometric Raman imaging and will have applications in drug discovery and biomedical research.
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