Evidence map›Paper›PMID 36924494›Full record

ArticleCell reports2023

MFGE8 links absorption of dietary fatty acids with catabolism of enterocyte lipid stores through HNF4γ-dependent transcription of CES enzymes.

Ritwik Datta, Mohammad A Gholampour, Christopher D Yang, Regan Volk, Sinan Lin, Michael J Podolsky, Thomas Arnold, Florian Rieder, Balyn W Zaro, Michael Verzi and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Ritwik DattaCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Mohammad A GholampourCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Christopher D YangCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Regan VolkCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Sinan LinDepartment of Gastroenterology, Hepatology and Nutrition, Digestive Diseases and Surgery Institute, Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44106, USA.
Michael J PodolskyCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Thomas ArnoldDepartment of Pediatrics, University of California, San Francisco, San Francisco, CA 94158, USA.
Florian RiederDepartment of Gastroenterology, Hepatology and Nutrition, Digestive Diseases and Surgery Institute, Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44106, USA.
Balyn W ZaroCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Michael VerziRutgers University, New Brunswick, NJ 08854, USA.
Richard LehnerDepartment of Pediatrics, University of Alberta, Edmonton, AB T6G 1C9, Canada.
Nada AbumradWashington University School of Medicine in St. Louis, St. Louis, MO 63110, USA.
Carlos O LizamaCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
Kamran AtabaiCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94158, USA; Lung Biology Center, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: kamran.atabai@ucsf.edu.
University of California, San Francisco · USCleveland Clinic Lerner College of Medicine · USRutgers, The State University of New Jersey · USUniversity of Alberta · CAWashington University in St. Louis · US

Funding

Washington University Nutrition Obesity Research CenterP30DK056341 · NIDDK · WASHINGTON UNIVERSITY · PI Dominic N Reeds · 1999 to 2026
$30.2M
Research Training in Chemistry and Chemical BiologyT32GM145460 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Jason E Gestwicki · 2022 to 2026
$3.1M
Septins in intestinal fibrosisR01DK132038 · NIDDK · CLEVELAND CLINIC LERNER COM-CWRU · PI Andrei Ivanovich Ivanov, Florian Rieder · 2023 to 2026
$2.5M
Integrin regulation of insulin sensitivityR01DK125310 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ATABAI, KAMRAN · 2022 to 2025
$2.1M
Creeping fat and Crohn's disease associated stricturesR01DK123233 · NIDDK · CLEVELAND CLINIC LERNER COM-CWRU · PI RIEDER, FLORIAN · 2020 to 2024
$1.9M
Investigating the role of cell-mediated collagen turnover in regulating tissue fibrosisR01HL136377 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ATABAI, KAMRAN · 2018 to 2021
$1.9M
integrin-mediated regulation of enterocyte lipid homeostasisR01DK110098 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ATABAI, KAMRAN · 2016 to 2019
$1.4M
Acquisition of Covaris E220 and Sciclone G3 systems for high throughput sequencinS10OD010786 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI COMAI, LUCA · 2012 to 2012
$311k
NHLBI NIH HHS R01 HL136377NIDDK NIH HHS P30 DK056341NIDDK NIH HHS R01 DK110098NIDDK NIH HHS R01 DK123233NIDDK NIH HHS R01 DK125310NIDDK NIH HHS R01 DK132038NIGMS NIH HHS T32 GM145460NIH HHS S10 OD010786
6 · The paper itself

Abstract

Enterocytes modulate the extent of postprandial lipemia by storing dietary fats in cytoplasmic lipid droplets (cLDs). We have previously shown that the integrin ligand MFGE8 links absorption of dietary fats with activation of triglyceride (TG) hydrolases that catabolize cLDs for chylomicron production. Here, we identify CES1D as the key hydrolase downstream of the MFGE8-αvβ5 integrin pathway that regulates catabolism of diet-derived cLDs. Mfge8 knockout (KO) enterocytes have reduced CES1D transcript and protein levels and reduced protein levels of the transcription factor HNF4γ. Both Ces1d and Hnf4γ KO mice have decreased enterocyte TG hydrolase activity coupled with retention of TG in cLDs. Mechanistically, MFGE8-dependent fatty acid uptake through CD36 stabilizes HNF4γ protein level; HNF4γ then increases Ces1d transcription. Our work identifies a regulatory network that regulates the severity of postprandial lipemia by linking dietary fat absorption with protein stabilization of a transcription factor that increases expression of hydrolases responsible for catabolizing diet-derived cLDs.

Indexed as

Dietary FatsEnterocytesAnimalsAntigens, SurfaceFatty AcidsHepatocyte Nuclear Factor 4HydrolasesLipid DropletsLipid MetabolismMiceMilk ProteinsTranscription FactorsTriglyceridesAntigens, SurfaceDietary FatsFatty AcidsHepatocyte Nuclear Factor 4Hnf4g protein, mouseHydrolasesMfge8 protein, mouseMilk ProteinsTranscription FactorsTriglyceridesCES1CP: MetabolismCP: Molecular biologydietary lipidsenterocytesHNF4γintegrinslipid dropletsMFGE8postprandial lipemiatriglyceride hydrolases

Identifiers

PMID36924494
PMCPMC10138282
OpenAlexW4324354010

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.