Evidence map›Paper›PMID 36923872›Full record

ArticleHeliyon2023

An artificialed protein corona coating the surface of magnetic nanoparicles:a simple and efficient method for label antibody.

Penghua Zhao, Xiaoyan Huang, Yaping Li, Xueping Huo, Qing Feng, Xiangrong Zhao, Cuixiang Xu, Jianhua Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Penghua ZhaoResearch Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Xiaoyan HuangShaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Yaping LiResearch Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Xueping HuoResearch Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Qing FengResearch Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Xiangrong ZhaoResearch Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Cuixiang XuResearch Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Jianhua WangResearch Center of Cell Immunological Engineering and Technology of Shaanxi Province, Central Lab of Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Shanxi Medical University · CNShaanxi Provincial People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Protein Corona (PC) of nanoparticles is a structure which composed of one or more layers of proteins adsorbed on the surface of nanomaterials, and the formation of PC is a universal process of spontaneous randomness. We take advantage of the formation principle of the PC, developed a simple and efficient method for label protein to nanoparticles. Methods: The artificialed protein corona (APC) on the surface of nanoparticles was synthesized via the artificialed methods of desolvation aggregation and crosslinking with control. Results: The dosage of precipitator and the ratio of protein to magnetic nanoparticles (MNPs)(particle size: 3 nm) were optimized, and the core-shell nanoparticles with narrow particle size (particle size: 10 nm) distribution were obtained. The MNPs with APC were characterized by transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Additionally, a hemolysis test on prepared MNPs was conducted with APC. The presence of APC coating on the surface of MNPs showed an improving effect to reduce the cytotoxicity. Cellular toxicity of MNPs with APC was also investigated on HFF1 cell lines. And the cells survival in the presence of APC coated MNPs and display neither reduced metabolism nor cytostatic effect. The functional test of the MNPs with APC showed that proteins can be modified and labeled onto magnetic nanoparticles and retain their original activity. Conclusions: This marking method is gentle and effective. And the properties of the APC propose MNPs as a promising candidate for multifunctional biomedical applications.

Indexed as

Antibody coated MNPsArtificialed protein corona(APC)BiocompatibilityCore shellMagnetic nanoparticles(MNPs)

Identifiers

PMID36923872
PMCPMC10008981
OpenAlexW4322615204

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.