Evidence map›Paper›PMID 36922971›Full record

ArticleFrontiers in microbiology2023

Characterization of surface-exposed structural loops as insertion sites for foreign antigen delivery in calicivirus-derived VLP platform.

Mirosława Panasiuk, Milena Chraniuk, Karolina Zimmer, Lilit Hovhannisyan, Vasil Krapchev, Grażyna Peszyńska-Sularz, Magdalena Narajczyk, Jan Węsławski, Agnieszka Konopacka, Beata Gromadzka

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In one paragraph

Article in Frontiers in microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Mirosława PanasiukDepartment of In Vitro Studies, Institute of Biotechnology and Molecular Medicine, Gdańsk, Poland.
Milena ChraniukDepartment of In Vitro Studies, Institute of Biotechnology and Molecular Medicine, Gdańsk, Poland.
Karolina ZimmerIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
Lilit HovhannisyanDepartment of In Vitro Studies, Institute of Biotechnology and Molecular Medicine, Gdańsk, Poland.
Vasil KrapchevIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
Grażyna Peszyńska-SularzTri-City Central Animal Laboratory Research and Service Center, Medical University of Gdańsk, Gdańsk, Poland.
Magdalena NarajczykLaboratory of Electron Microscopy, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.
Jan WęsławskiIntercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland.
Agnieszka KonopackaDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdańsk, Gdańsk, Poland.
Beata GromadzkaDepartment of In Vitro Studies, Institute of Biotechnology and Molecular Medicine, Gdańsk, Poland.
Gdańsk Medical University · PLInternational Institute of Molecular and Cell Biology · PLUniversity of Bielsko-Biała · PLUniversity of Gdańsk · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chimeric virus-like particles (cVLPs) show great potential in improving public health as they are safe and effective vaccine candidates. The capsid protein of caliciviruses has been described previously as a self-assembling, highly immunogenic delivery platform. The ability to significantly induce cellular and humoral immunity can be used to boost the immune response to low immunogenic foreign antigens displayed on the surface of VLPs. Capsid proteins of caliciviruses despite sequence differences share similar architecture with structural loops that can be genetically modified to present foreign epitopes on the surface of cVLPs. Here, based on the VP1 protein of norovirus (NoV), we investigated the impact of the localization of the epitope in different structural loops of the P domain on the immunogenicity of the presented epitope. In this study, three distinct loops of NoV VP1 protein were genetically modified to present a multivalent influenza virus epitope consisting of a tandem repeat of M2/NP epitopes. cVLPs presenting influenza virus-conserved epitopes in different localizations were produced in the insect cells and used to immunize BALB/c mice. Specific reaction to influenza epitopes was compared in sera from vaccinated mice to determine whether the localization of the foreign epitope has an impact on the immunogenicity.

Indexed as

caliciviruschimeric virus-like particlesforeign antigenimmunogenicityinfluenza A virusnorovirusvaccine developmentVLP platform

Identifiers

PMID36922971
PMCPMC10010390
OpenAlexW4322492187

What OpenQuestion holds

Textfull text, public
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.