Evidence map›Paper›PMID 36922514›Full record

ArticleNPJ Regenerative medicine2023

Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis.

Kristen M Stearns-Reider, Michael R Hicks, Katherine G Hammond, Joseph C Reynolds, Alok Maity, Yerbol Z Kurmangaliyev, Jesse Chin, Adam Z Stieg, Nicholas A Geisse, Sophia Hohlbauch and 9 more

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in NPJ Regenerative medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 5 institutions in 1 country.

Kristen M Stearns-ReiderDepartment of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Michael R HicksDepartment of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Katherine G HammondDepartment of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Joseph C ReynoldsDepartment of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0002-1931-4814
Alok MaityDepartment of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Yerbol Z KurmangaliyevInstitute for Quantitative and Computational Biology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Jesse ChinDepartment of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Adam Z StiegCalifornia NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0001-7312-9364
Nicholas A GeisseCuri Bio, Seattle, WA, 98121, USA.
Sophia HohlbauchAsylum Research, An Oxford Instruments Company, Santa Barbara, CA, 93117, USA.
Stefan KaemmerPark Systems, 3040 Olcott St, Santa Clara, CA, 95054, USA.
Lauren R SchmittDepartment of Biochemistry and Molecular Genetics, University of Colorado, Denver, Aurora, CO, 80045, USA.
Thanh T PhamDepartment of Biochemistry and Molecular Genetics, University of Colorado, Denver, Aurora, CO, 80045, USA.
Ken YamauchiEli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0003-2563-7545
Bennett G NovitchEli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Roy WollmanDepartment of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0003-3865-2605
Kirk C HansenDepartment of Biochemistry and Molecular Genetics, University of Colorado, Denver, Aurora, CO, 80045, USA.
April D PyleDepartment of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0001-6122-1649
Rachelle H CrosbieDepartment of Integrative Biology and Physiology, University of California, Los Angeles, Los Angeles, CA, 90095, USA. rcrosbie@physci.ucla.edu.ORCID http://orcid.org/0000-0001-6630-6713
University of California, Los Angeles · USQB3 · USUniversity of Colorado Denver · USCalifornia NanoSystems Institute · USOxford Instruments (United States) · US

Funding

University of Colorado Cancer Center Support Grant - Lung Cancer Patient-Derived Xenografts with Autologous Human Immune SystemsP30CA046934 · NCI · UNIVERSITY OF COLORADO DENVER · PI James V Degregori · 1988 to 2026
$117.0M
UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
Training and education CoreU54AR052646 · NIAMS · UNIVERSITY OF PENNSYLVANIA · PI MCNALLY, ELIZABETH M, SWEENEY, H LEE · 2005 to 2020
$24.1M
Role of Complement-Driven Pulmonary Vascular Inflammation in PHP01HL152961 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI DELANEY, CASSIDY A · 2020 to 2024
$14.1M
Structure-Function Analysis of SarcospanR01AR048179 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROSBIE, RACHELLE HOPE · 2001 to 2023
$6.9M
Reprogramming and Directed Differentiation of Skeletal Muscle Cells from hPSCs.R01AR064327 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI April D Pyle · 2013 to 2026
$6.4M
Understanding and Improving Therapies for the Muscular Dystrophies through Noninvasive BiomarkersP50AR052646 · NIAMS · UNIVERSITY OF FLORIDA · PI WALTER, GLENN · 2021 to 2024
$6.2M
Institutional Career Development CoreKL2TR001416 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI CAIOZZO, VINCENT JAMES, DEMETRIOU, MICHAEL · 2015 to 2023
$5.6M
Muscle Cell Biology, Pathophysiology, and TherapeuticsT32AR065972 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Rachelle Hope Crosbie, THOMAS A. RANDO · 2016 to 2026
$3.9M
Restoration of muscle cell adhesion to treat cardiomyopathy in muscular dystrophyR01HL126204 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROSBIE, RACHELLE HOPE · 2016 to 2019
$1.5M
Quadrupole-Orbitrap Mass SpectrometerS10OD021641 · OD · UNIVERSITY OF COLORADO DENVER · PI HANSEN, KIRK C · 2017 to 2017
$600k
NCATS NIH HHS UL1 TR000124NCI NIH HHS P30 CA046934NHLBI NIH HHS R01 HL126204NIAMS NIH HHS P50 AR052646NIAMS NIH HHS R01 AR048179NIAMS NIH HHS R01 AR064327NIAMS NIH HHS T32 AR065972NIAMS NIH HHS U54 AR052646
6 · The paper itself

Abstract

We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) myoscaffolds that can be repopulated with various cell types to interrogate cell-ECM interactions. Using this platform, we investigated whether fibrotic ECM scarring affected human skeletal muscle progenitor cell (SMPC) functions that are essential for myoregeneration. SMPCs exhibited robust adhesion, motility, and differentiation on healthy muscle-derived myoscaffolds. All SPMC interactions with fibrotic myoscaffolds from dystrophic muscle were severely blunted including reduced motility rate and migration. Furthermore, SMPCs were unable to remodel laminin dense fibrotic scars within diseased myoscaffolds. Proteomics and structural analysis revealed that excessive collagen deposition alone is not pathological, and can be compensatory, as revealed by overexpression of sarcospan and its associated ECM receptors in dystrophic muscle. Our in vivo data also supported that ECM remodeling is important for SMPC engraftment and that fibrotic scars may represent one barrier to efficient cell therapy.

Identifiers

PMID36922514
PMCPMC10017766
OpenAlexW4327680675

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.