ArticleMicrobial cell factories2023
Engineering Streptomyces albulus to enhance ε-poly-L-lysine production by introducing a polyphosphate kinase-mediated ATP regeneration system.
Article in Microbial cell factories, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 29 citations in OpenAlex.
- Multi-omics-guided metabolic engineering of Limosilactobacillus reuteri for high-level 3-hydroxypropionaldehyde production from glucose.Microbial cell factories · 2026Article
- Construction of engineered Streptomyces albulus by increasing ATP and NADH supply and efficient production of ε-poly-L-lysine from sweet potato powder.World journal of microbiology & biotechnology · 2026Article
- Enzymatic and microbial routes to bioplastics: The green chemistry frontier of biopolymers.FEBS open bio · 2026Review
- Identification of a distinct family of polyphosphate kinases relevant to energy homeostasis in Crenarchaeota.The Journal of biological chemistry · 2026Article
- Quantitative decoding of coupled carbon and energy metabolism in Pseudomonas putida for lignin carbon utilization.Communications biology · 2025Article
- Reconstitution of Methionine Cycle With ATP Regeneration for Whole-Cell Catalysis of Creatine Production in Engineered Escherichia coli.Microbial biotechnology · 2025Article
- Adaptive evolution and reverse engineering to explore the low pH tolerance mechanisms ofApplied and environmental microbiology · 2025Article
- Genomic and Metabolomic Analyses ofMicroorganisms · 2025Article
- Enhanced ε-Poly-L-Lysine Production inBiomolecules · 2024Article
- Metabolic engineering of an industrial bacteriumSynthetic and systems biotechnology · 2024Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
backgroundε-Poly-L-lysine (ε-PL) is a natural and safe food preservative that is mainly produced by filamentous and aerobic bacteria Streptomyces albulus. During ε-PL biosynthesis, a large amount of ATP is used for the polymerization of L-lysine. A shortage of intracellular ATP is one of the major factors limiting the increase in ε-PL production. In previous studies, researchers have mainly tried to increase the oxygen supply to enhance intracellular ATP levels to improve ε-PL production, which can be achieved through the use of two-stage dissolved oxygen control, oxygen carriers, heterologous expression of hemoglobin, and supplementation with auxiliary energy substrates. However, the enhancement of the intracellular ATP supply by constructing an ATP regeneration system has not yet been considered.
resultsIn this study, a polyphosphate kinase (PPK)-mediated ATP regeneration system was developed and introduced into S. albulus to successfully improve ε-PL production. First, polyP:AMP phosphotransferase (PAP) from Acinetobacter johnsonii was selected for catalyzing the conversion of AMP into ADP through an in vivo test. Moreover, three PPKs from different microbes were compared by in vitro and in vivo studies with respect to catalytic activity and polyphosphate (polyP) preference, and PPK2B
conclusionsIn this study, we proposed and developed a PPK-mediated ATP regeneration system in S. albulus for the first time and significantly enhanced ε-PL production. The study provides an efficient approach to improve the production of not only ε-PL but also other ATP-driven metabolites.
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