Evidence map›Paper›PMID 36918634›Full record

ArticleScientific reports2023

Molecular testing devices for on-site detection of E. coli in water samples.

Carlos Manzanas, Elise Morrison, Young S Kim, Morteza Alipanah, George Adedokun, Shouguang Jin, Todd Z Osborne, Z Hugh Fan

Open access · goldFull text read
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Development of a Platform forAerosol science and technology : the journal of the American Association for Aerosol Research · 2026
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Carlos ManzanasInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA.
Elise MorrisonDepartment of Environmental Engineering Sciences, University of Florida, P.O. Box 116580, Gainesville, FL, 32611, USA. elise.morrison@essie.ufl.edu.
Young S KimDepartment of Molecular Genetics and Microbiology, University of Florida, PO Box 100266, Gainesville, FL, 32610, USA.
Morteza AlipanahInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA.
George AdedokunInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA.
Shouguang JinDepartment of Molecular Genetics and Microbiology, University of Florida, PO Box 100266, Gainesville, FL, 32610, USA.
Todd Z OsborneWhitney Laboratory of Marine Bioscience, University of Florida, P.O. Box 116580, St. Augustine, FL, 32080, USA. osbornet@ufl.edu.
Z Hugh FanInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA. hfan@ufl.edu.
University of Florida · USWhitney Museum of American Art · US

Funding

Multiplexed Detection of Mosquito-Borne Viruses at the Point-of-CareR01AI155735 · NIAID · UNIVERSITY OF FLORIDA · PI FAN, Z. HUGH · 2021 to 2024
$1.5M
NIAID NIH HHS R01 AI155735NIH HHS R01AI155735
6 · The paper itself

Abstract

Escherichia coli (E. coli) cells are present in fecal materials that can be the main source for disease-causing agents in water. As a result, E. coli is recommended as a water quality indicator. We have developed an innovative platform to detect E. coli for monitoring water quality on-site by integrating paper-based sample preparation with nucleic acid isothermal amplification. The platform carries out bacterial lysis and DNA enrichment onto a paper pad through ball-based valves for fluid control, with no need of laboratory equipment, followed by loop-mediated isothermal amplification (LAMP) in a battery-operated coffee mug, and colorimetric detection. We have used the platform to detect E. coli in environmental water samples in about 1 h, with a limit of quantitation of 0.2 CFU/mL, and 3 copies per reaction. The platform was confirmed for detecting multiple E. coli strains, and for water samples of different salt concentrations. We validated the functions of the platform by analyzing recreational water samples collected near the Atlantic Ocean that contain different concentrations of salt and bacteria.

Indexed as

Escherichia coliNucleic Acid Amplification TechniquesAtlantic OceanBacteria

Identifiers

PMID36918634
PMCPMC10013241
OpenAlexW4324311613

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read18
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.