ArticlePlant biotechnology journal2023
Depletion of the NbCORE receptor drastically improves agroinfiltration productivity in older Nicotiana benthamiana plants.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Current Understanding of Cell-Surface Immune Receptors for MAMPs and Plant Parasites in the Solanaceae Family.Molecular plant pathology · 2026Review
- Plant chloroplasts enable high-yield and cost-effective production of the engineered bacterial enzyme CueO-MacHis as a technical protein via transient expression inFrontiers in plant science · 2026Article
- Transcriptomic analysis revealed distinct transcriptional responses inFrontiers in plant science · 2026Article
- Nicotiana benthamiana's Responses to Agroinfiltration, a Treasure Grove of New Avenues to Improve Protein Yields in Plant Molecular Farming.Plant biotechnology journal · 2026Review
- Rhizobium rhizogenes A4-derived strains mediate hyper-efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants.Plant biotechnology journal · 2026Article
- Tomato Roots Exhibit Development-Specific Responses to Bacterial-Derived Peptides.Plant, cell & environment · 2025Article
- Immunity gene silencing increases transient protein expression in Nicotiana benthamiana.Plant biotechnology journal · 2025Article
- Quantitative dissection of Agrobacterium T-DNA expression in single plant cells reveals density-dependent synergy and antagonism.Nature plants · 2025Article
- Plant pattern recognition receptors: from evolutionary insight to engineering.Nature reviews. Genetics · 2025Review
- Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025Review
- Plant Pattern recognition receptors: Exploring their evolution, diversification, and spatiotemporal regulation.Current opinion in plant biology · 2024Article
- Extracellular plant subtilases dampen cold-shock peptide elicitor levels.Nature plants · 2024Article
- The perception and evolution of flagellin, cold shock protein and elongation factor Tu from vector-borne bacterial plant pathogens.Molecular plant pathology · 2024Article
- Improving transient protein expression in agroinfiltrated Nicotiana benthamiana.The New phytologist · 2024Review
- Improving transient expression inFrontiers in plant science · 2024Article
- Effects of different light conditions on transient expression and biomass inOpen life sciences · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 3 countries.
Funding
Abstract
Nicotiana benthamiana is increasingly used for transient gene expression to produce antibodies, vaccines, and other pharmaceutical proteins but transient gene expression is low in fully developed, 6-8-week old plants. This low gene expression is thought to be caused by the perception of the cold shock protein (CSP) of Agrobacterium tumefaciens. The CSP receptor is contested because both NbCSPR and NbCORE have been claimed to perceive CSP. Here, we demonstrate that CSP perception is abolished in 6-week-old plants silenced for NbCORE but not NbCSPR. Importantly, older NbCORE-silenced plants support a highly increased level of GFP fluorescence and protein upon agroinfiltration. The drastic increase in transient protein production in NbCORE-depleted plants offers new opportunities for molecular farming, where older plants with larger biomass can now be used for efficient protein expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.