Evidence map›Paper›PMID 36917225›Full record

ArticleBiology of reproduction2023

Establishment and characterization of oviductal organoids from farm and companion animals†.

Edwina F Lawson, Arnab Ghosh, Victoria Blanch, Christopher G Grupen, Robert John Aitken, Rebecca Lim, Hannah R Drury, Mark A Baker, Zamira Gibb, Pradeep S Tanwar

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 22 citations in OpenAlex.

  1. Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026
    Review
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  9. Stem Cells and Wildlife Conservation.Advances in experimental medicine and biology · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Edwina F LawsonPriority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.
Arnab GhoshGlobal Centre for Gynaecological Diseases, University of Newcastle, Callaghan, NSW, Australia.
Victoria BlanchGlobal Centre for Gynaecological Diseases, University of Newcastle, Callaghan, NSW, Australia.
Christopher G GrupenSydney School of Veterinary Science, Faculty of Science, University of Sydney, Camden, NSW, Australia.
Robert John AitkenPriority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.
Rebecca LimSchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia.
Hannah R DrurySchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia.
Mark A BakerPriority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.
Zamira GibbPriority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW, Australia.
Pradeep S TanwarGlobal Centre for Gynaecological Diseases, University of Newcastle, Callaghan, NSW, Australia.
University of Newcastle Australia · AUHunter Medical Research Institute · AUUniversity of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Organoid technology has provided a unique opportunity to study early human development and decipher various steps involved in the pathogenesis of disease. The technology is already used in clinics to improve human patient outcomes. However, limited knowledge of the methodologies required to establish organoid culture systems in domestic animals has slowed the advancement and application of organoid technology in veterinary medicine. This is particularly true for the field of reproduction and the application of assisted reproductive technologies (ART). Here, we have developed a platform to grow oviductal organoids from five domestic species-bovine, porcine, equine, feline, and canine. The organoids were grown progressively from single cells derived from the enzymatic digestion of freshly collected infundibular/fimbrial samples. The addition of WNT, TGFβ, BMP, ROCK, and Notch signaling pathway activators or inhibitors to the organoid culture medium suggested remarkable conservation of the molecular signals involved in oviductal epithelial development and differentiation across species. The gross morphology of organoids from all the domestic species was initially similar. However, some differences in size, complexity, and growth rate were subsequently observed and described. After 21 days, well-defined and synchronized motile ciliated cells were observed in organoids. Histopathologically, oviductal organoids mimicked their respective native tissue. In summary, we have carried out a detailed cross-species comparison of oviductal organoids, which would be valuable in advancing our knowledge of oviduct physiology and, potentially, help in increasing the success of ART.

Indexed as

OrganoidsPetsAnimalsCatsCattleCell DifferentiationDogsFallopian TubesFarmsFemaleHorsesHumansSwinedisease modelingequinefertilityorganoidsoviduct

Identifiers

PMID36917225
PMCPMC10266943
OpenAlexW4324129010

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.