ArticleMolecular plant pathology2023
Site-specific analysis reveals candidate cross-kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley-powdery mildew interaction.
Article in Molecular plant pathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Cross-kingdom RNA in plant-microbe interactions: from molecular interactions to next-generation crop protection.Stress biology · 2026Review
- Fungal MicroRNA-Like RNAs Facilitate Convergent Attack on a Host Immune Signalling Network.Plant, cell & environment · 2026Article
- FungiSNC: An integrated database for fungi small non-coding RNAs based on high-throughput sequencing.Journal of advanced research · 2026Article
- Stage-Specific RNA Turnover Drives Small RNA Dynamics inPlant direct · 2026Article
- Plant-derived, stress-responsive microRNA mimics reduce powdery mildew infections of barley.Frontiers in plant science · 2026Article
- Review
- SmithRNAs: A Common Feature among Metazoa.Genome biology and evolution · 2025Article
- Vesicular and non-vesicular extracellular small RNAs direct gene silencing in a plant-interacting bacterium.Nature communications · 2025Article
- rRFtargetDB: a database of Ago1-mediated targets of ribosomal RNA fragments.RNA (New York, N.Y.) · 2025Article
- Practical advice for extracellular vesicle isolation in plant-microbe interactions: Concerns, considerations, and conclusions.Journal of extracellular vesicles · 2024Article
- Interkingdom Communication via Extracellular Vesicles: Unraveling Plant and Pathogen Interactions and Its Potential for Next-Generation Crop Protection.Microorganisms · 2024Review
- Comprehensive profiling of rRNA-derived small RNAs inMethodsX · 2024Article
- The extracellular vesicle proteomes of Sorghum bicolor and Arabidopsis thaliana are partially conserved.Plant physiology · 2024Article
- Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.Current opinion in plant biology · 2023Review
- Article
- A perspective on cross-kingdom RNA interference in mutualistic symbioses.The New phytologist · 2023Review
- Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.Nature communications · 2023Article
- Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.bioRxiv : the preprint server for biology · 2023Article
- Extracellular vesicles: cross-organismal RNA trafficking in plants, microbes, and mammalian cells.Extracellular vesicles and circulating nucleic acids · 2023Article
- Site-specific analysis reveals candidate cross-kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley-powdery mildew interaction.Molecular plant pathology · 2023Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The establishment of host-microbe interactions requires molecular communication between both partners, which may involve the mutual transfer of noncoding small RNAs. Previous evidence suggests that this is also true for powdery mildew disease in barley, which is caused by the fungal pathogen Blumeria hordei. However, previous studies lacked spatial resolution regarding the accumulation of small RNAs upon host infection by B. hordei. Here, we analysed site-specific small RNA repertoires in the context of the barley-B. hordei interaction. To this end, we dissected infected leaves into separate fractions representing different sites that are key to the pathogenic process: epiphytic fungal mycelium, infected plant epidermis, isolated haustoria, a vesicle-enriched fraction from infected epidermis, and extracellular vesicles. Unexpectedly, we discovered enrichment of specific 31-33-base 5'-terminal fragments of barley 5.8S ribosomal RNA in extracellular vesicles and infected epidermis, as well as particular B. hordei transfer RNA fragments in haustoria. We describe canonical small RNAs from both the plant host and the fungal pathogen that may confer cross-kingdom RNA interference activity. Interestingly, we found first evidence of phased small interfering RNAs in B. hordei, a feature usually attributed to plants, which may be associated with the posttranscriptional control of fungal coding genes, pseudogenes, and transposable elements. Our data suggest a key and possibly site-specific role for cross-kingdom RNA interference and noncoding RNA fragments in the host-pathogen communication between B. hordei and its host barley.
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