Evidence map›Paper›PMID 36916500›Full record

ArticleMolecular oncology2023

Disclosing quantitative RT-PCR raw data during manuscript submission: a call for action.

Andreas Untergasser, Jan Hellemans, Michael W Pfaffl, Jan M Ruijter, Maurice J B van den Hoff, Mihnea P Dragomir, Douglas Adamoski, Sandra Martha Gomes Dias, Rui Manuel Reis, Manuela Ferracin and 19 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular oncology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Andreas UntergasserZentrum für Molekulare Biologie der Universität Heidelberg, Germany.
Jan HellemansCellCarta, Zwijnaarde, Belgium.
Michael W PfafflDivision of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany.
Jan M RuijterDepartment of Medical Biology, Amsterdam UMC, location AMC, University of Amsterdam, The Netherlands.
Maurice J B van den HoffDepartment of Medical Biology, Amsterdam UMC, location AMC, University of Amsterdam, The Netherlands.
Mihnea P DragomirInstitute of Pathology, Berlin Institute of Health, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Germany.
Douglas AdamoskiBrazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil.
Sandra Martha Gomes DiasBrazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil.
Rui Manuel ReisMolecular Oncology Research Center, Barretos Cancer Hospital, Brazil.ORCID 0000-0002-9639-7940
Manuela FerracinDepartment of Medical and Surgical Sciences (DIMEC), University of Bologna, Italy.ORCID 0000-0002-1595-6887
Emmanuel Dias-NetoLaboratory of Medical Genomics, Centro Internacional de Pesquisa, AC Camargo Cancer Center, São Paulo, Brazil.
Ian MarshNew South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Narellan, Australia.
Mikael KubistaTATAA Biocenter AB, Göteborg, Sweden.
Muller FabbriCenter for Cancer and Immunology Research, Children's National Hospital, Washington, DC, USA.
Ajay GoelDepartment of Molecular Diagnostics and Experimental Therapeutics, Beckman Research Institute of City of Hope, Monrovia, CA, USA.ORCID 0000-0003-1396-6341
Ondřej SlabýDepartment of Biology, Faculty of Medicine, Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Erik KnutsenDepartment of Medical Biology, Faculty of Health Sciences, UiT-The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0002-4903-0150
Baoqing ChenDepartment of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
Massimo NegriniLaboratory for Technologies of Advanced Therapies (LTTA), Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Italy.
Koshi MimoriDepartment of Surgery, Kyushu University Beppu Hospital, Japan.
Martin PichlerDivision of Oncology, Department of Internal Medicine, Medical University of Graz, Austria.
Maria PapatriantafyllouEditorial Manager, Molecular Oncology, Cambridge, UK.
Simone AnfossiDepartment of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Thomas D SchmittgenDepartment of Pharmaceutics, University of Florida College of Pharmacy, Gainesville, FL, USA.
Jim HuggettNational Measurement Laboratory, LGC, Teddington, UK.
Stephen BustinMedical Technology Research Centre, Anglia Ruskin University, Chelmsford, Essex, UK.
Jo VandesompeleCellCarta, Zwijnaarde, Belgium.
George A CalinDepartment of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.ORCID 0000-0002-7427-0578
HEROIC (tHe initiativE gRoup On qRT dIsClosure) Consortium

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.

Indexed as

RNAHumansReal-Time Polymerase Chain ReactionReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNAaccuracyquantificationRNART-qPCR

Identifiers

PMID36916500
PMCPMC10158759

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.