ArticleProceedings of the National Academy of Sciences of the United States of America2023
Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 36 citations in OpenAlex.
- A longitudinal single-nucleus transcriptomic atlas of bovine placentation reveals dynamic cellular hierarchies and regulatory programs.Genome biology · 2026Article
- Cross-species insights into placental evolution and diseases at the single-cell resolution.Nature communications · 2026Article
- Article
- Directed differentiation of bovine trophoblast stem cells: A useful in vitro model for placenta development.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Placental adaptation to maternal environment in domestic animals.The Journal of reproduction and development · 2026Review
- Update on endogenous retroviruses in cattle placenta.The Journal of reproduction and development · 2026Review
- Trophoblast differentiation in the bovine placenta.The Journal of reproduction and development · 2026Review
- Trophoblast Fusion Deficiency in Placenta-Related Pregnancy Disorders.Advances in experimental medicine and biology · 2026Review
- Cross-species identification of conserved cell-type specific mechanisms during early placenta development in ruminants.Scientific reports · 2025Article
- Transcriptomic Profiling of Peripheral Blood Identifies Candidate Genes for Early Pregnancy Diagnosis in Sika Deer.Animals : an open access journal from MDPI · 2025Article
- Analyzing the relationship of RNA and DNA methylation with gene expression.Genome biology · 2025Article
- Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024Review
- Article
- Single-Cell Sequencing Technology in Ruminant Livestock: Challenges and Opportunities.Current issues in molecular biology · 2024Review
- Spatially resolved transcriptomic profiling of placental development in dairy cow.Zoological research · 2024Article
- Article
- A review of single-cell transcriptomics and epigenomics studies in maternal and child health.Epigenomics · 2024Review
- Single-cell insights into epithelial morphogenesis in the neonatal mouse uterus.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Progressive Exaptation of Endogenous Retroviruses in Placental Evolution in Cattle.Biomolecules · 2023Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ruminants have a semi-invasive placenta, which possess highly vascularized placentomes formed by maternal endometrial caruncles and fetal placental cotyledons and required for fetal development to term. The synepitheliochorial placenta of cattle contains at least two trophoblast cell populations, including uninucleate (UNC) and binucleate (BNC) cells that are most abundant in the cotyledonary chorion of the placentomes. The interplacentomal placenta is more epitheliochorial in nature with the chorion developing specialized areolae over the openings of uterine glands. Of note, the cell types in the placenta and cellular and molecular mechanisms governing trophoblast differentiation and function are little understood in ruminants. To fill this knowledge gap, the cotyledonary and intercotyledonary areas of the mature day 195 bovine placenta were analyzed by single nuclei analysis. Single-nuclei RNA-seq analysis found substantial differences in cell type composition and transcriptional profiles between the two distinct regions of the placenta. Based on clustering and cell marker gene expression, five different trophoblast cell types were identified in the chorion, including proliferating and differentiating UNC and two different types of BNC in the cotyledon. Cell trajectory analyses provided a framework for understanding the differentiation of trophoblast UNC into BNC. The upstream transcription factor binding analysis of differentially expressed genes identified a candidate set of regulator factors and genes regulating trophoblast differentiation. This foundational information is useful to discover essential biological pathways underpinning the development and function of the bovine placenta.
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