Evidence map›Paper›PMID 36912688›Full record

ArticleJournal of AOAC International2023

Extensive Evaluation of a Method for Quantitative Measurement of Aflatoxins B1 and M1 in Animal Urine Using High-Performance Liquid Chromatography with Fluorescence Detection.

Xiangwei Du, Dwayne E Schrunk, Paula M Imerman, John Tahara, Andriy Tkachenko, Jake Guag, Renate Reimschuessel, Wilson K Rumbeiha

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of AOAC International, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Xiangwei DuVeterinary Medical Diagnostic Laboratory, Department of Biomedical Sciences, University of Missouri, 901 East Campus Loop, Columbia, MO 65211, USA.ORCID 0000-0003-4445-8921
Dwayne E SchrunkVeterinary Diagnostic Laboratory, Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 1850 Christensen Dr, Ames, IA 50011, USA.
Paula M ImermanVeterinary Diagnostic Laboratory, Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, 1850 Christensen Dr, Ames, IA 50011, USA.
John TaharaCalifornia Animal Health and Food Safety Laboratory System, Toxicology Laboratory, University of California, Davis, CA 95616, USA.
Andriy TkachenkoUnited States Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Rd, Laurel, MD 20708, USA.ORCID 0000-0002-0048-9243
Jake GuagUnited States Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Rd, Laurel, MD 20708, USA.
Renate ReimschuesselUnited States Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Rd, Laurel, MD 20708, USA.ORCID 0000-0002-3979-8617
Wilson K RumbeihaDepartment of Molecular Biosciences, University of California, Davis, CA 95616, USA.ORCID 0000-0002-2370-1817
Center for Veterinary Medicine · USIowa State University · USCalifornia Department of Food and Agriculture · USMissouri College · USUniversity of California, Davis · US

Funding

ISU Vet-LIRN Collaborative Validation of Testing Methods on Animal Diagnostic SpeU18FD005006 · FDA · IOWA STATE UNIVERSITY · PI GAUGER, PHILLIP · 2013 to 2017
$495k
FDA HHS U18 FD005006
6 · The paper itself

Abstract

backgroundAflatoxins (AFs) are common feed contaminants and are one of the common causes of toxin-related pet food poisoning and recalls.

objectiveCurrently, there are no validated methods for the detection and quantitation of AFs in biological matrices to diagnose AF exposure in live animals. Following a successful intra-laboratory method development to quantify AFB1 and AFM1 in animal urine by HPLC with fluorescence detection (HPLC-FLD), the present study was conducted to extensively evaluate the method performance in an unbiased manner using blinded samples.

methodsThe evaluation included two stages. First, the performance was verified in the method-originating laboratory in a single-laboratory blinded method test (BMT-S) trial followed by a multi-laboratory blinded method test (BMT-M) trial.

resultsIn both trials, accuracy, repeatability, and reproducibility were satisfactory confirming the relatively good ruggedness and robustness of the method and ensuring that it will perform as expected if used by other laboratories in the future.

conclusionsWe extensively evaluated the performance of a quantitative method to detect AFB1 and AFM1 in animal urine by HPLC-FLD by two different laboratories in two separate BMT-S and BMT-M trials. Both BMT results demonstrated the satisfactory accuracy and precision of the method. It is now available to be adopted by other diagnostic laboratories for purposes of diagnosing AF intoxication in animals. HIGHLIGHTS: A simple urine-based diagnostic test method using HPLC-FLD that originated in a single laboratory now has passed a multi-laboratory evaluation and is now available to be shared with other diagnostic laboratories for purposes of diagnosing AF intoxication in animals so better treatment can be rendered.

Indexed as

Aflatoxin B1AflatoxinsAnimalsChromatography, High Pressure LiquidFood ContaminationReproducibility of ResultsAflatoxin B1Aflatoxins

Identifiers

PMID36912688
PMCPMC10156413
OpenAlexW4324018062

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.