ArticleInfection and immunity2023
Identification of Multiple Iron Uptake Mechanisms in Enterococcus faecalis and Their Relationship to Virulence.
Article in Infection and immunity, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 23 citations in OpenAlex.
- Iron, Biofilm, and Colorectal Cancer: Mechanistic Insights Into Enterococcus faecalis Pathogenesis.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2026Review
- Characterization of a novel cell wall-associated nucleotidase of Enterococcus faecalis that degrades extracellular c-di-AMP.PLoS pathogens · 2026Article
- Review
- The effects of dietary iron supplementation on bacterial infections inbioRxiv : the preprint server for biology · 2026Article
- Metal uptake systems underpinInfection and immunity · 2026Article
- The effects of dietary iron supplementation on bacterial infections in Manduca sexta larval hemolymph.PloS one · 2026Article
- The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification inVirulence · 2025Article
- Maternal anemia during pregnancy and its association with the gut microbiota profile in pregnant and postpartum women, and their infants: a cohort study.The American journal of clinical nutrition · 2025Article
- Bioengineered gut bacterium synthesizing levodopa alleviates motor deficits in models of Parkinson's disease.Cell host & microbe · 2025Article
- Pharmacological strategies for targeting biofilms in otorhinolaryngologic infections and overcoming antimicrobial resistance (Review).Biomedical reports · 2025Review
- Article
- Insights into ecology, pathogenesis, and biofilm formation ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- A Novel Heme-Degrading Enzyme that Regulates Heme and Iron Homeostasis and Promotes Virulence inbioRxiv : the preprint server for biology · 2025Article
- The potential impact of iron supply on the development of starvedFrontiers in microbiology · 2025Article
- Evaluation of bacterial adherence and biofilm development on an anodized stainless-steel surface for the prevention of osteosynthesis-associated infections.Journal of bone and joint infection · 2025Article
- Metals in the gut: microbial strategies to overcome nutritional immunity in the intestinal tract.Metallomics : integrated biometal science · 2024Review
- Radiotracers for in situ infection imaging: Experimental considerations for in vitro microbial uptake of gallium-68-labeled siderophores.Diagnostic microbiology and infectious disease · 2024Article
- Clinical sequelae of gut microbiome development and disruption in hospitalized preterm infants.Cell host & microbe · 2024Article
- The distribution and antibiotic-resistant characteristics and risk factors of pathogens associated with clinical biliary tract infection in humans.Frontiers in microbiology · 2024Article
- Trivalent immunization with metal-binding proteins confers protection against enterococci in a mouse infection model.FEMS microbes · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Among the unfavorable conditions bacteria encounter within the host is restricted access to essential trace metals such as iron. To overcome iron deficiency, bacteria deploy multiple strategies to scavenge iron from host tissues, with abundant examples of iron acquisition systems being implicated in bacterial pathogenesis. Yet the mechanisms utilized by the major nosocomial pathogen Enterococcus faecalis to maintain intracellular iron balance are poorly understood. In this study, we conducted a systematic investigation to identify and characterize the iron acquisition mechanisms of E. faecalis and to determine their contribution to virulence. Bioinformatic analysis and literature surveys revealed that E. faecalis possesses three conserved iron uptake systems. Through transcriptomics, we discovered two novel ABC-type transporters that mediate iron uptake. While inactivation of a single transporter had minimal impact on the ability of E. faecalis to maintain iron homeostasis, inactivation of all five systems (Δ5Fe strain) disrupted intracellular iron homeostasis and considerably impaired cell growth under iron deficiency. Virulence of the Δ5Fe strain was generally impaired in different animal models but showed niche-specific variations in mouse models, leading us to suspect that heme can serve as an iron source to E. faecalis during mammalian infections. Indeed, heme supplementation restored growth of Δ5Fe under iron depletion and virulence in an invertebrate infection model. This study revealed that the collective contribution of five iron transporters promotes E. faecalis virulence and that the ability to acquire and utilize heme as an iron source is critical to the systemic dissemination of E. faecalis.
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Registered trials
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