Evidence map›Paper›PMID 36910576›Full record

ArticleAmerican journal of molecular biology2012

SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA deadenylation.

Yajun Cui, Yueh-Chin Chiang, Palaniswamy Viswanathan, Darren J Lee, Clyde L Denis

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yajun CuiDepartment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, USA.
Yueh-Chin ChiangDepartment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, USA.
Palaniswamy ViswanathanDepartment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, USA.
Darren J LeeDepartment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, USA.
Clyde L DenisDepartment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, USA.
University of New Hampshire · US

Funding

YEAST GENES INVOLVED IN GENERAL TRANSCRIPTIONAL CONTROLR01GM041215 · NIGMS · UNIVERSITY OF NEW HAMPSHIRE · PI DENIS, CLYDE L · 1990 to 2003
$1.2M
Factors affecting mRNA deadenylationR15GM087684 · NIGMS · UNIVERSITY OF NEW HAMPSHIRE · PI DENIS, CLYDE L · 2009 to 2010
$297k
NIGMS NIH HHS R01 GM041215NIGMS NIH HHS R15 GM087684
6 · The paper itself

Abstract

The CCR4-NOT complex has been shown to have multiple roles in mRNA metabolism, including that of transcriptional elongation, mRNA transport, and nuclear exosome function, but the primary function of CCR4 and CAF1 is in the deadenylation and degradation of cytoplasmic mRNA. As previous genetic analysis supported an interaction between SPT5, known to be involved in transcriptional elongation, and that of CCR4, the physical association of SPT5 with CCR4 was examined. A two-hybrid screen utilizing the deadenylase domain of CCR4 as a bait identified SPT5 as a potential interacting protein. SPT5 at its physiological concentration was shown to immunoprecipitate CCR4 and CAF1, and in vitro purified SPT5 specifically could bind to CAF1 and the deadenylase domain of CCR4. We additionally demonstrated that mutations in

Indexed as

CCR4-NOT ComplexDeadenylationmRNA DegradationSPT5/SPT4

Identifiers

PMID36910576
PMCPMC9997522
OpenAlexW2111036454

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.