Evidence map›Paper›PMID 36910509›Full record

ArticleAccess microbiology2023

Evaluation of five commercial DNA extraction kits using

Shannon H C Eagle, James Robertson, D Patrick Bastedo, Kira Liu, John H E Nash

Abstract read
In one paragraph

Article in Access microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025
    Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shannon H C EagleNational Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.ORCID https://orcid.org/0000-0002-6643-8799
James RobertsonNational Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada.ORCID https://orcid.org/0000-0002-8534-5694
D Patrick BastedoNational Microbiology Laboratory, Public Health Agency of Canada, Toronto, Ontario, Canada.ORCID https://orcid.org/0000-0002-7698-7994
Kira LiuPatented Medicine Prices Review Board, Ottawa, Ontario, Canada.
John H E NashNational Microbiology Laboratory, Public Health Agency of Canada, Toronto, Ontario, Canada.ORCID https://orcid.org/0000-0003-2925-8710

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxford Nanopore long-read sequencing offers advantages over Illumina short reads for the identification and characterization of bacterial pathogens for outbreak detection and surveillance activities within a diagnostic public health laboratory context. Compared to Illumina, Nanopore is more cost-effective for small batches, has a lower capital cost and has a faster turnaround time, in addition to the ability to assemble complete bacterial genomes. The quantity and quality of DNA required for Nanopore sequencing are greater than for Illumina, and the DNA extraction methods recommended for obtaining high-molecular-weight DNA are different from those typically used in diagnostic laboratories. Using a

Indexed as

bacterial genomicsDNA extractionslong-read sequencingOxford Nanopore sequencingpublic healthwhole-genome sequencing

Identifiers

PMID36910509
PMCPMC9996181

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.