Evidence map›Paper›PMID 36909610›Full record

ArticleResearch square2023

FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators.

Osha Roopnarine, Samantha L Yuen, Andrew R Thompson, Lauren N Roelike, Robyn T Rebbeck, Phillip A Bidwell, Courtney C Aldrich, Razvan L Cornea, David D Thomas

Open access · greenFull text readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Osha RoopnarineUniversity of Minnesota.
Samantha L YuenUniversity of Minnesota.
Andrew R ThompsonUniversity of Minnesota.
Lauren N RoelikeUniversity of Minnesota.
Robyn T RebbeckUniversity of Minnesota.
Phillip A BidwellUniversity of Minnesota.
Courtney C AldrichUniversity of Minnesota.
Razvan L CorneaUniversity of Minnesota.
David D ThomasUniversity of Minnesota.
University of Minnesota System · US

Funding

Structural Dynamics of Cardiac Muscle Calcium ATPase RegulationR01HL139065 · NHLBI · UNIVERSITY OF MINNESOTA · PI Robyn T Rebbeck, David D Thomas · 2020 to 2026
$5.2M
Site-Directed Oxidative Modification of Muscle Protein Structural DynamicsR37AG026160 · NIA · UNIVERSITY OF MINNESOTA · PI THOMAS, DAVID D · 2011 to 2020
$4.5M
NHLBI NIH HHS R01 HL139065NIA NIH HHS R37 AG026160
6 · The paper itself

Abstract

We have used FRET-based biosensors in live cells, in a robust high-throughput screening (HTS) platform, to identify small-molecules that alter the structure and activity of the cardiac sarco/endoplasmic reticulum calcium ATPase (SERCA2a). Our primary aim is to discover drug-like small-molecule activators that improve SERCA’s function for the treatment of heart failure. We have previously demonstrated the use of an intramolecular FRET biosensor, based on human SERCA2a, by screening a small validation library using novel microplate readers that can detect the fluorescence lifetime or emission spectrum with high speed, precision, and resolution. Here we report results from a 50,000-compound screen using the same biosensor, with hit compounds functionally evaluated using Ca

Identifiers

PMID36909610
PMCPMC10002828
OpenAlexW4322626089

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.