Evidence map›Paper›PMID 36909485›Full record

ArticlebioRxiv : the preprint server for biology2023

Lipofuscin-like autofluorescence within microglia and its impact on studying microglial engulfment.

Jacob M Stillman, Francisco M Lopes, Jing-Ping Lin, Kevin Hu, Daniel S Reich, Dorothy P Schafer

Open access · greenFull text readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Jacob M StillmanDepartment of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Francisco M LopesDepartment of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Jing-Ping LinTranslational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Kevin HuTranslational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Daniel S ReichTranslational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Dorothy P SchaferDepartment of Neurobiology, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
National Institutes of Health · USUniversity of Massachusetts Chan Medical School · US

Funding

Microglia-dependent mechanisms governing neural circuit plasticityR01MH113743 · NIMH · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Dorothy Patricia Schafer · 2017 to 2026
$4.9M
How Do Synaptic Connections Change in Demyelinating Disease?R01NS117533 · NINDS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI SCHAFER, DOROTHY PATRICIA · 2021 to 2025
$2.2M
Dissecting the impact of senescence on microglia function and neurodegenerationRF1AG068281 · NIA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI SCHAFER, DOROTHY PATRICIA, ZHANG, HONG · 2020 to 2020
$1.7M
Training in the Molecular Basis of Autoimmunity and AutoinflammationT32AI132152 · NIAID · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Katherine A. Fitzgerald, Ann Marshak-Rothstein · 2018 to 2026
$1.6M
NIAID NIH HHS T32 AI132152NIA NIH HHS RF1 AG068281NIMH NIH HHS R01 MH113743NINDS NIH HHS R01 NS117533
6 · The paper itself

Abstract

Engulfment of cellular material and proteins is a key function for microglia, a resident macrophage of the central nervous system (CNS). Among the techniques used to measure microglial engulfment, confocal light microscopy has been used the most extensively. Here, we show that autofluorescence (AF), likely due to lipofuscin and typically associated with aging, can also be detected within microglial lysosomes in the young mouse brain by light microscopy. This lipofuscin-AF signal accumulates first within microglia and increases with age, but it is not exacerbated by amyloid beta-related neurodegeneration. We further show that this lipofuscin-AF signal within microglia can confound the interpretation of antibody-labeled synaptic material within microglia in young adult mice. Finally, we implement a robust strategy to quench AF in mouse, marmoset, and human brain tissue.

Identifiers

PMID36909485
PMCPMC10002639
OpenAlexW4323049190

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read28
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.