Evidence map›Paper›PMID 36909481›Full record

ArticlebioRxiv : the preprint server for biology2023

An interchangeable prion-like domain is required for Ty1 retrotransposition.

Sean L Beckwith, Emily J Nomberg, Abigail C Newman, Jeannette V Taylor, Ricardo C Guerrero, David J Garfinkel

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Sean L BeckwithDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Emily J NombergDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Abigail C NewmanDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
Jeannette V TaylorRobert P. Apkarian Integrated Electron Microscopy Core at Emory University, Atlanta, GA, 30322, USA.
Ricardo C GuerreroRobert P. Apkarian Integrated Electron Microscopy Core at Emory University, Atlanta, GA, 30322, USA.
David J GarfinkelDepartment of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA.
University of Georgia · USEmory University · US

Funding

Atlanta Clinical and Translational Science Institute (ACTSI) RenewalUL1TR000454 · NCATS · EMORY UNIVERSITY · PI STEPHENS, DAVID S · 2012 to 2016
$25.8M
Effectors of retrotransposon movementR01GM124216 · NIGMS · UNIVERSITY OF GEORGIA · PI GARFINKEL, DAVID J. · 2018 to 2021
$1.9M
FEI Transmission Electron Microscope with Tomography CapabilityS10RR025679 · NCRR · EMORY UNIVERSITY · PI SPEARMAN, PAUL W. · 2009 to 2009
$500k
Retrotransposon virus-like particle assemblyF32GM139247 · NIGMS · UNIVERSITY OF GEORGIA · PI BECKWITH, SEAN · 2020 to 2021
$134k
NCATS NIH HHS UL1 TR000454NCRR NIH HHS S10 RR025679NIGMS NIH HHS F32 GM139247NIGMS NIH HHS R01 GM124216
6 · The paper itself

Abstract

Retrotransposons and retroviruses shape genome evolution and can negatively impact genome function. Significance: Retrovirus-like retrotransposons help shape the genome evolution of their hosts and replicate within cytoplasmic particles. How their building blocks associate and assemble within the cell is poorly understood. Here, we report a novel pr ion-like d omain (PrLD) in the budding yeast retrotransposon Ty1 Gag protein that builds virus-like particles. The PrLD has similar sequence properties to prions and disordered protein domains that can drive the formation of assemblies that range from liquid to solid. We demonstrate that the Ty1 PrLD can function as a prion and that certain prion sequences can replace the PrLD and support Ty1 transposition. This interchangeable system is an effective platform to study additional disordered sequences in living cells.

Identifiers

PMID36909481
PMCPMC10002725
OpenAlexW4322487179

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.