Evidence map›Paper›PMID 36909433›Full record

ArticleFrontiers in plant science2023

Design of an artificial transcriptional system for production of high levels of recombinant proteins in tobacco (

Areum Yun, Joohyun Kang, Juhun Lee, Shi-Jian Song, Inhwan Hwang

Abstract read
In one paragraph

Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Areum YunDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Joohyun KangDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Juhun LeeDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Shi-Jian SongDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Inhwan HwangDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plants have recently received much attention as a means of producing recombinant proteins because they are easy to grow at a low cost and at a large scale. Although many plant protein expression systems have been developed, there remains a need for improved systems that deliver high yields of recombinant proteins. Transcription of the recombinant gene is a key step in increasing the yield of recombinant proteins. However, revealed strong promoters, terminators, and transcription factors that have been identified do not necessarily lead to high level production of recombinant proteins. Thus, in this study, a robust expression system was designed to produce high levels of recombinant protein consisting of a novel hybrid promoter, FM'M-UD, coupled with an artificial terminator, 3PRt. FM'M-UD contained fragments from three viral promoters (the promoters of

Indexed as

artificial transcription factorNicotiana benthamianarecombinant protein productionstrong promoterstrong terminator

Identifiers

PMID36909433
PMCPMC9995837

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.