Evidence map›Paper›PMID 36905455›Full record

ArticleMikrochimica acta2023

SERS-fluorescence dual-mode nanoprobe for the detection and imaging of Bax mRNA during apoptosis.

Xichi Lin, Chenbiao Li, Shan Xu, Zhouping Wang, Xiaoyuan Ma

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Xichi LinState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Chenbiao LiState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Shan XuState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Zhouping WangState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Xiaoyuan MaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China. maxy@jiangnan.edu.cn.
Jiangnan University · CNState Key Laboratory of Food Science and Technology · CN

Funding

Jiangsu Agriculture Science and Technology Innovation Fund CX (19)2005Social Development Fund Project of Wuxi N20201001
6 · The paper itself

Abstract

A dual-mode nanoprobe was constructed to detect Bax messenger RNA (mRNA), consisting of gold nanotriangles (AuNTs), a Cy5-modified recognition sequence, and a thiol-modified DNA sequence. Bax mRNA is one of the key pro-apoptotic factors in the apoptosis pathway. Raman enhancement and fluorescence quenching of the signal group Cy5 were performed using AuNTs as substrates. The thiol-modified nucleic acid chain is partially complementary to the Cy5-modified nucleic acid chain to form a double strand and is linked to the AuNTs by the Au-S bond. When Bax mRNA is present, the Cy5-modified strand specifically binds to it to form a more stable duplex, making Cy5 far away from AuNTs, and SERS signal is weakened while fluorescence signal is enhanced. The nanoprobe can be used for the quantitative detection of Bax mRNA in vitro. Combined with the high sensitivity of SERS and the visualization of fluorescence, this method has good specificity and can be used for in situ imaging and dynamic monitoring of Bax mRNA during deoxynivalenol (DON) toxin-induced apoptosis of HepG2 cells. DON plays a pathogenic role mainly by inducing cell apoptosis. The results confirmed that the proposed dual-mode nanoprobe has good versatility in various human cell lines.

Indexed as

ApoptosisSulfhydryl Compoundsbcl-2-Associated X ProteinCarbocyaninesCell Line, TumorFluorescenceHumansRNA, Messengerbcl-2-Associated X ProteinCarbocyaninescyanine dye 5RNA, MessengerSulfhydryl CompoundsBax mRNACell imagingDual-mode nanoprobeFunctional nucleic acidOptical detectionVomitoxin

Identifiers

PMID36905455
OpenAlexW4323921259

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.