Essentiality of the

Torben Lund, Maria Yohanna Kulkova, Rosa Jersie-Christensen, Tove Atlung

Full text read

In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

The hidden role of rhizospheric viruses in promoting nitrogen fixation in soils.Nature communications · 2026
Article
The iron-sulfur accelerator YgfZ modulates genome-wide IHF-binding dynamics to regulate replication initiation inFrontiers in microbiology · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Torben LundDepartment of Science and Environment, Roskilde University, Universitetsvej 1, 4000 Roskilde, Denmark.

Maria Yohanna KulkovaDepartment of Science and Environment, Roskilde University, Universitetsvej 1, 4000 Roskilde, Denmark.

Rosa Jersie-ChristensenDepartment of Science and Environment, Roskilde University, Universitetsvej 1, 4000 Roskilde, Denmark.

Tove AtlungDepartment of Science and Environment, Roskilde University, Universitetsvej 1, 4000 Roskilde, Denmark.ORCID 0000-0002-9324-4164

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzymes carrying Iron-Sulfur (Fe-S) clusters perform many important cellular functions and their biogenesis require complex protein machinery. In mitochondria, the IBA57 protein is essential and promotes assembly of [4Fe-4S] clusters and their insertion into acceptor proteins. YgfZ is the bacterial homologue of IBA57 but its precise role in Fe-S cluster metabolism is uncharacterized. YgfZ is needed for activity of the radical S-adenosyl methionine [4Fe-4S] cluster enzyme MiaB which thiomethylates some tRNAs. The growth of cells lacking YgfZ is compromised especially at low temperature. The RimO enzyme is homologous to MiaB and thiomethylates a conserved aspartic acid in ribosomal protein S12. To quantitate thiomethylation by RimO, we developed a bottom-up LC-MS

Indexed as

Escherichia coli ProteinsIron-Sulfur ProteinsEscherichia coliRibosomal ProteinsSulfurSulfurtransferasesEscherichia coli ProteinsIron-Sulfur ProteinsMiaB protein, E coliRibosomal Proteinsribosomal protein S12SulfurSulfurtransferases4Fe-4S clusterbottom-up proteomicsRadical SAMS12 D88targeted LC-MS2

Identifiers

PMID36902159

DOI10.3390/ijms24054728

PMCPMC10002905

What OpenQuestion holds

Textfull text, public

LicenceCC BY

measurements read49

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.