Evidence map›Paper›PMID 36901991›Full record

ArticleInternational journal of molecular sciences2023

Skeletal Muscle-Derived Exosomal miR-146a-5p Inhibits Adipogenesis by Mediating Muscle-Fat Axis and Targeting GDF5-PPARγ Signaling.

Mengran Qin, Lipeng Xing, Jiahan Wu, Shulei Wen, Junyi Luo, Ting Chen, Yaotian Fan, Jiahao Zhu, Lekai Yang, Jie Liu and 10 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Mengran QinGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.ORCID 0000-0001-5990-6550
Lipeng XingGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Jiahan WuGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Shulei WenGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Junyi LuoGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Ting ChenGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Yaotian FanGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Jiahao ZhuGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Lekai YangGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Jie LiuGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.ORCID 0000-0002-5028-8589
Jiali XiongGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Xingping ChenJiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Canjun ZhuGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Songbo WangGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.ORCID 0000-0001-9190-9401
Lina WangGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.ORCID 0000-0001-8133-3349
Gang ShuGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Qingyan JiangGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Yongliang ZhangGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.
Jiajie SunGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.ORCID 0000-0003-1992-154X
Qianyun XiGuangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Livestock and Poultry Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, No. 483 Wushan Road, Guangzhou 510642, China.

Funding

National Natural Science Foundation of China 31872435National Natural Science Foundation of China 32072714National Natural Science Foundation of China 32072812National Natural Science Foundation of China 32072814
6 · The paper itself

Abstract

Skeletal muscle-fat interaction is essential for maintaining organismal energy homeostasis and managing obesity by secreting cytokines and exosomes, but the role of the latter as a new mediator in inter-tissue communication remains unclear. Recently, we discovered that miR-146a-5p was mainly enriched in skeletal muscle-derived exosomes (SKM-Exos), 50-fold higher than in fat exosomes. Here, we investigated the role of skeletal muscle-derived exosomes regulating lipid metabolism in adipose tissue by delivering miR-146a-5p. The results showed that skeletal muscle cell-derived exosomes significantly inhibited the differentiation of preadipocytes and their adipogenesis. When the skeletal muscle-derived exosomes co-treated adipocytes with miR-146a-5p inhibitor, this inhibition was reversed. Additionally, skeletal muscle-specific knockout miR-146a-5p (mKO) mice significantly increased body weight gain and decreased oxidative metabolism. On the other hand, the internalization of this miRNA into the mKO mice by injecting skeletal muscle-derived exosomes from the Flox mice (Flox-Exos) resulted in significant phenotypic reversion, including down-regulation of genes and proteins involved in adipogenesis. Mechanistically, miR-146a-5p has also been demonstrated to function as a negative regulator of peroxisome proliferator-activated receptor γ (PPARγ) signaling by directly targeting growth and differentiation factor 5 (GDF5) gene to mediate adipogenesis and fatty acid absorption. Taken together, these data provide new insights into the role of miR-146a-5p as a novel myokine involved in the regulation of adipogenesis and obesity via mediating the skeletal muscle-fat signaling axis, which may serve as a target for the development of therapies against metabolic diseases, such as obesity.

Indexed as

ExosomesMicroRNAsAdipogenesisAdipose TissueAnimalsGrowth Differentiation Factor 5MiceMuscle, SkeletalObesityPPAR gammaGdf5 protein, mouseGrowth Differentiation Factor 5MicroRNAsPPAR gammaadipogenesiscrosstalkexosomesGDF5miR-146a-5pPPARγskeletal muscle

Identifiers

PMID36901991
PMCPMC10003660

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.