Evidence map›Paper›PMID 36899374›Full record

ArticleMicrobial cell factories2023

Unravel the regulatory mechanism of Yrr1p phosphorylation in response to vanillin stress in Saccharomyces cerevisiae.

Weiquan Zhao, Xinning Wang, Bolun Yang, Ying Wang, Zailu Li, Xiaoming Bao

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Weiquan ZhaoState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology Shandong Academy of Sciences, Jinan, 250353, China.
Xinning WangState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology Shandong Academy of Sciences, Jinan, 250353, China. xinningwang@sdu.edu.cn.
Bolun YangState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology Shandong Academy of Sciences, Jinan, 250353, China.
Ying WangState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology Shandong Academy of Sciences, Jinan, 250353, China.
Zailu LiState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology Shandong Academy of Sciences, Jinan, 250353, China.
Xiaoming BaoState Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology Shandong Academy of Sciences, Jinan, 250353, China. baoxm@qlu.edu.cn.
Shandong Academy of Sciences · CNQilu University of Technology · CN

Funding

ESI Cultivation Discipline of Biology and Biochemistry, and Qilu University of Technology No. 202015National Key Research and Development Program of China 2018YFB1501401State Key Laboratory of Biobased Material and Green Papermaking of Qilu University of Technology(Shandong Academy of Sciences) ZZ20190306the Key innovation Project of Qilu University of Technology (Shandong Academy of Sciences) 2022JBZ01-06the Key R&D Project of Shandong Province 2019JZZY010906the Major Program of Shandong Province Natural Science Foundation ZR2018ZB0209the National Natural Science Foundation of China 31870063the Shandong Provincial Natural Science Foundation ZR2019BC080the Special Foundation for International Cooperation Research QLUTGJHZ2018009
6 · The paper itself

Abstract

Improving the resistance of Saccharomyces cerevisiae to vanillin, derived from lignin, will benefit the design of robust cell factories for lignocellulosic biorefining. The transcription factor Yrr1p mediates S. cerevisiae resistance to various compounds. In this study, eleven predicted phosphorylation sites were mutated, among which 4 mutants of Yrr1p, Y134A/E and T185A/E could improve vanillin resistance. Both dephosphorylated and phosphorylated mutations at Yrr1p 134 and 185 gathered in the nucleus regardless of the presence or absence of vanillin. However, the phosphorylated mutant Yrr1p inhibited target gene expression, while dephosphorylated mutants promoted expression. Transcriptomic analysis showed that the dephosphorylated Yrr1p T185 mutant, under vanillin stress, upregulated ribosome biogenesis and rRNA processing. These results demonstrate the mechanism by which Yrr1p phosphorylation regulates the expression of target genes. The identification of key phosphorylation sites in Yrr1p offers novel targets for the rational construction of Yrr1p mutants to improve resistance to other compounds.

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsBenzaldehydesPhosphorylationBenzaldehydesSaccharomyces cerevisiae ProteinsvanillinPhosphorylation sitesRegulation activitySaccharomyces cerevisiaeSubcellular locationVanillin resistanceYrr1p

Identifiers

PMID36899374
PMCPMC10007725
OpenAlexW4323921061

What OpenQuestion holds

Textfull text, public
LicenceCC BY
measurements read38
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.