Evidence map›Paper›PMID 36899354›Full record

ArticleCancer cell international2023

Hypoxia-responsive circular RNA circAAGAB reduces breast cancer malignancy by activating p38 MAPK and sponging miR-378 h.

Kuan-Yi Lee, Chia-Ming Liu, Li-Han Chen, Chien-Yueh Lee, Tzu-Pin Lu, Li-Ling Chuang, Liang-Chuan Lai

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kuan-Yi LeeGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Chia-Ming LiuGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Li-Han ChenInstitute of Fisheries Science, College of Life Science, National Taiwan University, Taipei, Taiwan.
Chien-Yueh LeeMaster Program for Biomedical Engineering, College of Biomedical Engineering, China Medical University, Taichung, Taiwan.
Tzu-Pin LuInstitute of Epidemiology and Preventive Medicine, Department of Public Health, National Taiwan University, Taipei, Taiwan.
Li-Ling ChuangSchool of Physical Therapy and Graduate Institute of Rehabilitation Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan. lchuang@gap.cgu.edu.tw.
Liang-Chuan LaiGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, Taiwan. llai@ntu.edu.tw.
National Taiwan University · TWChang Gung University · TWChina Medical University · TW

Funding

Ministry of Science and Technology 109-2320-B-002-016-MY3
6 · The paper itself

Abstract

backgroundBreast cancer is a prevalent disease in women, with high prevalence worldwide. The hypoxic microenvironment of solid tumors develops during the progress of carcinogenesis and leads to greater malignancy and treatment resistance. Recently, accumulating evidence indicates that non-coding RNAs, such as circular RNAs (circRNAs), play a pivotal role in altering cellular functions. However, the underlying mechanisms of circRNAs in breast cancer are still unclear. Therefore, the purpose of this study was to investigate the role of a tumor-suppressive circRNA, circAAGAB, in breast cancer by assuming down-regulation of circAAGAB under hypoxia and the properties of a tumor suppressor.

methodsFirstly, circAAGAB was identified from expression profiling by next generation sequencing. Next, the stability of circAAGAB increased by interacting with the RNA binding protein FUS. Moreover, cellular and nuclear fractionation showed that most circAAGAB resided in the cytoplasm and that it up-regulated KIAA1522, NKX3-1, and JADE3 by sponging miR-378 h. Lastly, the functions of circAAGAB were explored by identifying its down-stream genes using Affymetrix microarrays and validated by in vitro assays.

resultsThe results showed that circAAGAB reduced cell colony formation, cell migration, and signaling through p38 MAPK pathway, as well as increased radiosensitivity.

conclusionThese findings suggest that the oxygen-responsive circAAGAB acts as a tumor suppressor in breast cancer, and may contribute to the development of a more specific therapeutic regimen for breast cancer.

Indexed as

Breast cancercircAAGABHypoxiamiRNA spongep38 MAPKRadiosensitivityTumor suppressor

Identifiers

PMID36899354
PMCPMC10007766
OpenAlexW4323920670

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.