Evidence map›Paper›PMID 36899129›Full record

ArticleArchives of virology2023

Characterization and complete genome sequence analysis of the novel phage RPZH3 infecting Ralstonia solanacearum.

Zhijian Lin, Gang Gu, Changjiang Chen, Ting Zhou, Fangping Hu, Xueqing Cai

Abstract read
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Article in Archives of virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhijian LinCollege of Plant Protection, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, China.
Gang GuInstitute of Tobacco Science, Fujian Provincial Tobacco Company, Fuzhou, Fujian, 350013, China.
Changjiang ChenCollege of Computer and Information Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
Ting ZhouInstitute of Tobacco Science, Fujian Provincial Tobacco Company, Fuzhou, Fujian, 350013, China.
Fangping HuCollege of Plant Protection, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, China.
Xueqing CaiCollege of Plant Protection, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, China. caixq90@163.com.ORCID http://orcid.org/0000-0003-0686-3854

Funding

Fujian Provincial Company of China Tobacco MYC 2017006Fujian Provincial Company of China Tobacco MYC 2022005National Key Research and Development Program of China 2017YFD0201106-02the Guiding projects in Fujian Province 2022N0004
6 · The paper itself

Abstract

A novel lytic Ralstonia phage, RPZH3, was isolated from the soil of a tobacco field via a double agar overlay plaque assay. The phage has an icosahedral head 75 ± 5 nm in diameter with a short tail about 15 ± 5 nm in length. It was able to infect 18 out of 30 tested strains of R. solanacearum isolated from tobacco, sweet potato, tomato, pepper, and eggplant. The latent period of the phage was 80 min, and the burst period was 60 min with a burst size of about 27 pfu/cell. The phage was stable at pH 4-12 at 28°C, and it was also stable at temperatures from 45°C to 60°C at pH 7.0. The complete genome of phage RPZH3 consists of 65,958 bp, with a GC content of 64.93%. The genome contains 93 open reading frames (ORFs) and encodes a tRNA for cysteine. Nucleotide sequence alignment and phylogenetic analysis indicated that RPZH3 is a new member of the genus Gervaisevirus belonging to the class Caudoviricetes.

Indexed as

BacteriophagesRalstonia solanacearumGenome, ViralOpen Reading FramesPhylogenySequence Analysis

Identifiers

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.