ArticleHeliyon2023
Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues.
Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Assessing Mitochondrial Respiratory Complex-Associated Function From Previously Frozen Mouse Placental Tissue.Bio-protocol · 2026Article
- An investigation into the sex dependence of post-reperfusion cardiac mitochondrial function and redox balance in chronically stressed rats.Physiological reports · 2025Article
- Analysis of mitochondrial Complex I activity and ATP hydrolysis capacity of ATP synthase in developing rat brains using frozen tissues.microPublication biology · 2025Article
- Subzero project: comparing trace element profiles of enriched mitochondria fractions from frozen and fresh liver tissue.Analytical and bioanalytical chemistry · 2024Article
- Mitochondrial dysfunction in brain tissues and Extracellular Vesicles Fragile X-associated tremor/ataxia syndrome.Annals of clinical and translational neurology · 2024Article
- Mitochondrial respiratory capacity is not altered in aging rat brains with or without memory impairment.microPublication biology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Studying mitochondrial respiration capacity is essential for gaining insights into mitochondrial functions. In frozen tissue samples, however, our ability to study mitochondrial respiration is restricted by damage elicited to the inner mitochondrial membranes by freeze-thaw cycles. We developed an approach that combines multiple assays and is tailored towards assessing mitochondrial electron transport chain and ATP synthase in frozen tissues. Using small amounts of frozen tissue, we systematically analyzed the quantity as well as activity of both the electron transport chain complexes and ATP synthase in rat brains during postnatal development. We reveal a previously little-known pattern of increasing mitochondrial respiration capacity with brain development. In addition to providing proof-of-principle evidence that mitochondrial activity changes during brain development, our study details an approach that can be applicable to many other types of frozen cell or tissue samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.