Evidence map›Paper›PMID 36894953›Full record

ArticlePlant methods2023

Corn360: a method for quantification of corn kernels.

Samantha Gillette, Lu Yin, Penny M A Kianian, Wojciech P Pawlowski, Changbin Chen

Abstract read
In one paragraph

Article in Plant methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Samantha Gillette *School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA.
Lu Yin *School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA.
Penny M A KianianPepsiCo Inc., 210 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA.
Wojciech P PawlowskiSchool of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
Changbin ChenSchool of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA. cchen347@asu.edu.

Funding

National Science Foundation, United States IOS-1546792
6 · The paper itself

Abstract

backgroundThe rapidly advancing corn breeding field calls for high-throughput methods to phenotype corn kernel traits to estimate yield and to study their genetic inheritance. Most of the existing methods are reliant on sophisticated setup, expertise in statistical models and programming skills for image capturing and analysis.

resultsWe demonstrated a portable, easily accessible, affordable, panoramic imaging capturing system called Corn360, followed by image analysis using freely available software, to characterize total kernel count and different patterned kernel counts of a corn ear. The software we used did not require programming skills and utilized Artificial Intelligence to train a model and to segment the images of mixed patterned corn ears. For homogeneously patterned corn ears, our results showed accuracies of 93.7% of total kernel count compared to manual counting. Our method allowed to save an average of 3 min 40 s per image. For mixed patterned corn ears, our results showed accuracies of 84.8% or 61.8% of segmented kernel counts. Our method has the potential to greatly decrease counting time per image as the number of images increases. We also demonstrated a case of using Corn360 to count different categories of kernels on a mixed patterned corn ear resulting from a cross of sweet corn and sticky corn and showed that starch:sweet:sticky segregated in a 9:4:3 ratio in its F2 population.

conclusionsThe panoramic Corn360 approach enables for a portable low-cost high-throughput kernel quantification. This includes total kernel quantification and quantification of different patterned kernels. This can allow for quick estimate of yield component and for categorization of different patterned kernels to study the inheritance of genes controlling color and texture. We demonstrated that using the samples resulting from a sweet × sticky cross, the starchiness, sweetness and stickiness in this case were controlled by two genes with epistatic effects. Our achieved results indicate Corn360 can be used to effectively quantify corn kernels in a portable and cost-efficient way that is easily accessible with or without programming skills.

Indexed as

CornHigh-throughput phenotypingImage analysisKernel colorKernel textureLow-cost

Identifiers

PMID36894953
PMCPMC9996904

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.