ArticleNature communications2023
A general highly efficient synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Harnessing fluorescent probes for molecular diagnosis and theranostics of atherosclerosis.Drug delivery · 2026Review
- Bis-Tetrazine Fluorogenic (Silicon)-Rhodamine Dyes for Live-Cell Labeling.Journal of the American Chemical Society · 2026Article
- A Highly Selective, Cell-Permeable Fluorescent Probe for Imaging Histone Deacetylase 6 in Live Cells.Journal of the American Chemical Society · 2026Article
- A comprehensive review of mitochondrial fluorescent probes: Design principles, fluorophore engineering, and functional parameter sensing.Materials today. Bio · 2026Review
- A Contrastive-Learning-Based Pre-Training Framework for Optical Property Prediction of Low-Data Rhodamines with Interpretable Multitask Graph Neural Networks.Molecules (Basel, Switzerland) · 2026Article
- Chemical fluorophores for fluorescence lifetime imaging.Chemical Society reviews · 2026Review
- Regioisomer-controlled red-shifted DNA probes for imaging of living tissues.bioRxiv : the preprint server for biology · 2025Article
- Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells.Nature communications · 2025Article
- Visualizing the chronicle of multiple cell fates using a near-IR dual-RNA/DNA-targeting probe.Science advances · 2025Article
- Fluorescent labeling strategies for molecular bioimaging.Biophysical reports · 2025Review
- Advances in Natural-Product-Based Fluorescent Agents and Synthetic Analogues for Analytical and Biomedical Applications.Bioengineering (Basel, Switzerland) · 2024Review
- Nano-fluorescence imaging: advancing lymphatic disease diagnosis and monitoring.Nano convergence · 2024Review
- Construction of Targeting-Peptide-Based Imaging Reagents and Their Application in Bioimaging.Chemical & biomedical imaging · 2024Review
- Article
- Optimized Red-Absorbing Dyes for Imaging and Sensing.Journal of the American Chemical Society · 2023Article
- Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
7 authors.
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Abstract
The development of live-cell fluorescence nanoscopy is powered by the availability of suitable fluorescent probes. Rhodamines are among the best fluorophores for labeling intracellular structures. Isomeric tuning is a powerful method for optimizing the biocompatibility of rhodamine-containing probes without affecting their spectral properties. An efficient synthesis pathway for 4-carboxyrhodamines is still lacking. We present a facile protecting-group-free 4-carboxyrhodamines' synthesis based on the nucleophilic addition of lithium dicarboxybenzenide to the corresponding xanthone. This approach drastically reduces the number of synthesis steps, expands the achievable structural diversity, increases overall yields and permits gram-scale synthesis of the dyes. We synthesize a wide range of symmetrical and unsymmetrical 4-carboxyrhodamines covering the whole visible spectrum and target them to multiple structures in living cells - microtubules, DNA, actin, mitochondria, lysosomes, Halo-tagged and SNAP-tagged proteins. The enhanced permeability fluorescent probes operate at submicromolar concentrations, allowing high-contrast STED and confocal microscopy of living cells and tissues.
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