ArticleFrontiers in cellular and infection microbiology2023
The value of next-generation metagenomic sequencing in pathogen detection of pleural effusions and ascites from children with sepsis.
Article in Frontiers in cellular and infection microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 6 citations in OpenAlex.
- Recent advances and clinical applications for the rapid detection of pathogenic bacteria in biological samples.Bioanalysis · 2025Review
- Epidemiological characteristics and trends in the diagnosis and treatment of fungal otitis externa in children.Translational pediatrics · 2025Article
- Diagnostic value of metagenomic next-generation sequencing combined by medical thoracoscopy surgery among infectious pleural effusion patients.BMC infectious diseases · 2025Article
- Evaluating the feasibility, sensitivity, and specificity of next-generation molecular methods for pleural infection diagnosis.Microbiology spectrum · 2025Observational
- Retrospective clinical and microbiologic analysis of metagenomic next-generation sequencing in the microbiological diagnosis of cutaneous infectious granulomas.Annals of clinical microbiology and antimicrobials · 2024Article
- Nanopore sequencing of infectious fluid is a promising supplement for gold-standard culture in real-world clinical scenario.Frontiers in cellular and infection microbiology · 2024Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
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Abstract
Objective: To investigate the diagnostic value of metagenomic next-generation sequencing (mNGS) using pleural effusion and ascites from children with sepsis. Methods: In this study, children with sepsis or severe sepsis and appeared pleural or peritoneal effusions were enrolled, of whom the pleural effusions or ascites and blood samples were conducted pathogen detection using both conventional and mNGS methods. The samples were divided into pathogen-consistent and pathogen-inconsistent groups based on the consistency of mNGS results from different sample types, and into exudate and transudate groups based on their pleural effusion and ascites properties. The pathogen positive rates, pathogen spectrum, consistency between different sample types, and clinical diagnosis consistency were compared between mNGS and conventional pathogen tests. Results: A total of 42 pleural effusions or ascites and 50 other type samples were collected from 32 children. The pathogen positive rate of the mNGS test was significantly higher than that of traditional methods (78.57% Conclusion: Compared to conventional methods, mNGS has great advantages in pathogen detection of pleural effusion and ascites samples. Moreover, consistent results of mNGS tests with different sample types provide more reference values in clinical diagnosis.
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