Evidence map›Paper›PMID 36890298›Full record

ArticleParasitology research2023

Molecular detection of Babesia spp. and Rickettsia spp. in coatis (Nasua nasua) and associated ticks from midwestern Brazil.

L Perles, W T G Barreto, G C de Macedo, A C Calchi, M Bezerra-Santos, J A Mendoza-Roldan, D Otranto, H M Herrera, D M Barros-Battesti, R Z Machado and 1 more

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.5field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Diversity of piroplasmids in wild animals in Brazil: a review.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2025
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

L PerlesVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, Jaboticabal, SP, 14884-900, Brazil.
W T G BarretoPost-Graduation of Ecology and Conservation, Mato Grosso Do Sul Federal University, Campo Grande, MS, 13471-410, Brazil.
G C de MacedoLaboratory of Parasitic Biology, Environmental Sciences and Farming Sustainability, Dom Bosco Catholic University, Campo Grande, MS, 13471-410, Brazil.
A C CalchiVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, Jaboticabal, SP, 14884-900, Brazil.
M Bezerra-SantosDepartment of Veterinary Medicine, University of Bari, Valenzano, Italy.
J A Mendoza-RoldanDepartment of Veterinary Medicine, University of Bari, Valenzano, Italy.
D OtrantoDepartment of Veterinary Medicine, University of Bari, Valenzano, Italy.
H M HerreraPost-Graduation of Ecology and Conservation, Mato Grosso Do Sul Federal University, Campo Grande, MS, 13471-410, Brazil.
D M Barros-BattestiVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, Jaboticabal, SP, 14884-900, Brazil.
R Z MachadoVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, Jaboticabal, SP, 14884-900, Brazil.
M R AndréVector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane, S/N, Zona Rural, Jaboticabal, SP, 14884-900, Brazil. mr.andre@unesp.br.
Universidade Estadual Paulista (Unesp) · BRUniversity of Bari Aldo Moro · ITUniversidade Católica Dom Bosco · BRUniversidade Federal de Mato Grosso do Sul · BR

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 303701/2021-8Conselho Nacional de Desenvolvimento Científico e Tecnológico 308768/2017-5Fundação de Amparo à Pesquisa do Estado de São Paulo 2019/15150-4Fundação de Amparo à Pesquisa do Estado de São Paulo 2020/12037-0NextGenerationEU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases PE00000007
6 · The paper itself

Abstract

Procyonids are reservoirs of many zoonotic infectious diseases, including tick-borne pathogens. The role of coatis (Nasua nasua) in the epidemiology of piroplasmids and Rickettsia has not been fully addressed in Brazil. To molecularly study these agents in coatis and associated ticks, animals were sampled in two urban areas in Midwestern Brazil. Blood (n = 163) and tick (n = 248) DNA samples were screened by PCR assays targeting the 18S rRNA and gltA genes of piroplasmids and Rickettsia spp., respectively. Positive samples were further molecularly tested targeting cox-1, cox-3, β-tubulin, cytB, and hsp70 (piroplasmid) and ompA, ompB, and htrA 17-kDa (Rickettsia spp.) genes, sequenced and phylogenetically analyzed. All coatis' blood samples were negative for piroplasmids, whereas five pools of ticks (2%) were positive for two different sequences of Babesia spp.. The first from Amblyomma sculptum nymphs was close (i.e., ≥ 99% nucleotide identity) to a Babesia sp. previously found in capybaras (Hydrochoerus hydrochaeris); the second from Amblyomma dubitatum nymphs and Amblyomma spp. larvae was identical (100% nucleotide identity) to a Babesia sp. detected in opossums (Didelphis albiventris) and associated ticks. Four samples (0.8%) were positive by PCR to two different Rickettsia spp. sequences, being the first from Amblyomma sp. larva identical to Rickettsia belli and the second from A. dubitatum nymph identical to Rickettsia species from Spotted Fever Group (SFG). The detection of piroplasmids and SFG Rickettsia sp. highlights the importance of Amblyomma spp. in the maintenance of tick-borne agents in urban parks where humans and wild and domestic animals are living in sympatry.

Indexed as

BabesiaIxodidaeProcyonidaeRickettsiaTicksAmblyommaAnimalsBrazilHumansOpossumsRodentiaAmblyomma dubitatumAmblyomma sculptumBabesia sp.SFG Rickettsia sp.Tick-borne pathogensZoonosis

Identifiers

PMID36890298
OpenAlexW4323652229

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.