Evidence map›Paper›PMID 36888783›Full record

ArticleRevista da Sociedade Brasileira de Medicina Tropical2023

Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus.

Blenda Fernandes, Emanoelle Fernandes Rutren La Santrer, Sônia Maria de Figueiredo, Fabiana Rocha-Silva, Cláudia Barbosa Assunção, Amanda Gabrielle Abreu, Iara Furtado Santiago, Susana Johann, Rachel Basques Caligiorne

Open access · diamondAbstract read
In one paragraph

Article in Revista da Sociedade Brasileira de Medicina Tropical, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Antimicrobial peptides from arthropod venoms exhibit activity againstThe journal of venomous animals and toxins including tropical diseases · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Blenda FernandesHospital Santa Casa de Belo Horizonte, Ensino e Pesquisa, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0002-0827-1134
Emanoelle Fernandes Rutren La SantrerHospital Santa Casa de Belo Horizonte, Ensino e Pesquisa, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0002-6415-7758
Sônia Maria de FigueiredoUniversidade Federal de Ouro Preto, Escola de Nutrição, Programa de Pós-Graduação em Saúde e Nutrição, Ouro Preto, MG, Brasil.ORCID http://orcid.org/0000-0001-8149-5110
Fabiana Rocha-SilvaHospital Santa Casa de Belo Horizonte, Ensino e Pesquisa, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0003-2698-9649
Cláudia Barbosa AssunçãoHospital Santa Casa de Belo Horizonte, Ensino e Pesquisa, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0001-5530-9310
Amanda Gabrielle AbreuHospital Santa Casa de Belo Horizonte, Ensino e Pesquisa, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0003-1251-1660
Iara Furtado SantiagoUniversidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Microbiologia, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0001-5324-4445
Susana JohannUniversidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Microbiologia, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0001-8068-1720
Rachel Basques CaligiorneHospital Santa Casa de Belo Horizonte, Ensino e Pesquisa, Belo Horizonte, MG, Brasil.ORCID http://orcid.org/0000-0001-7574-0984
Instituto de Ensino e Pesquisa Santa Casa · BRUniversidade Federal de Minas Gerais · BRUniversidade Federal de Ouro Preto · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR.

methodsA specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique.

resultsThree specific primers with 100% specificity for the Sporothrix genus were generated.

conclusionsPCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.

Indexed as

SporothrixSporotrichosisAnimalsBase SequenceHumansPolymerase Chain Reaction

Identifiers

PMID36888783
PMCPMC9991107
OpenAlexW4323343566

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.