Evidence map›Paper›PMID 36888609›Full record

ArticlePloS one2023

Development of a programmable magnetic agitation device to maintain colloidal suspension of cells during microfluidic syringe pump perfusion.

Tommy Puttrich, Steven O'Donnell, Sing-Wan Wong, Miiri Kotche, Anthony E Felder, Jae-Won Shin

Abstract read
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Periodic Flows in Microfluidics.Small (Weinheim an der Bergstrasse, Germany) · 2024
    Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tommy PuttrichThe Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, United States of America.
Steven O'DonnellThe Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, United States of America.
Sing-Wan WongThe Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, United States of America.
Miiri KotcheThe Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, United States of America.
Anthony E FelderThe Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, United States of America.
Jae-Won ShinThe Richard and Loan Hill Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, United States of America.ORCID 0000-0003-4823-6373

Funding

Encapsulation of mesenchymal stromal cells in engineered microgels for resolution of lung fibrosisR01HL141255 · NHLBI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI SHIN, JAE-WON · 2019 to 2023
$2.0M
Engineering microscale hydrogel deposition to direct single stem cell differentiationR01GM141147 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI SHIN, JAE-WON · 2021 to 2024
$1.9M
NHLBI NIH HHS R01 HL141255NIGMS NIH HHS R01 GM141147
6 · The paper itself

Abstract

Droplet-based microfluidic devices have been used to achieve homogeneous cell encapsulation, but cells sediment in a solution, leading to heterogeneous products. In this technical note, we describe automated and programmable agitation device to maintain colloidal suspensions of cells. We demonstrate that the agitation device can be interfaced with a syringe pump for microfluidic applications. Agitation profiles of the device were predictable and corresponded to device settings. The device maintains the concentration of cells in an alginate solution over time without implicating cell viability. This device replaces manual agitation, and hence is suitable for applications that require slow perfusion for a longer period of time in a scalable manner.

Indexed as

MicrofluidicsSyringesCell SurvivalMagnetic PhenomenaPerfusion

Identifiers

PMID36888609
PMCPMC9994697

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.