ArticleFEBS open bio2023
Fast and efficient molecule delivery into Euglena gracilis mediated by cell-penetrating peptide or dimethyl sulfoxide.
Article in FEBS open bio, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 4 citations in OpenAlex.
- Integrative framework for selecting gene delivery technologies in microalgae for synthetic biology.Molecular biology reports · 2026Review
- Review
- A chromosome-level genome assembly for the paramylon-producing microalga Euglena gracilis.Scientific data · 2024Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
This study describes the development of two methods for delivering exogenous materials into Euglena gracilis, a unicellular flagellate organism. We report that the use of Pep-1, a short cell-penetrating peptide (CPP), or dimethyl sulfoxide (DMSO) can mediate fast and efficient intracellular delivery of exogenous materials into E. gracilis, achieving cellular entry efficiency as high as 70-80%. However, compared with human cells, the penetration of this algal cell with CPP requires a much higher concentration of purified proteins. In addition, upon convenient treatment with DMSO, E. gracilis cells can efficiently adsorb exogenous proteins and DNA with 10% DMSO as the optimal concentration for Euglena cells. Our results provide more options for the E. gracilis transformation 'toolkit box' and will facilitate future molecular manipulations of this microalgal organism.
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Registered trials
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