Evidence map›Paper›PMID 36882950›Full record

ArticleMagnetic resonance in chemistry : MRC2023

Monitoring live mitochondrial metabolism in real-time using NMR spectroscopy.

G A Nagana Gowda, Vadim Pascua, John A Lusk, Natalie N Hong, Lin Guo, Jiyang Dong, Ian R Sweet, Daniel Raftery

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Magnetic resonance in chemistry : MRC, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Live Metabolomics with NMR.Analytical chemistry · 2026
    Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

G A Nagana GowdaNorthwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.ORCID 0000-0002-0544-7464
Vadim PascuaNorthwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.
John A LuskNorthwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.
Natalie N HongNorthwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.
Lin GuoNorthwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.
Jiyang DongNorthwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.ORCID 0000-0002-1064-6548
Ian R SweetDepartment of Medicine, University of Washington, Seattle, Washington, USA.
Daniel RafteryNorthwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.ORCID 0000-0003-2467-8118

Funding

PILOT STUDY--CLINICAL NUTRITION RESEARCHP30DK035816 · NIDDK · UNIVERSITY OF WASHINGTON · PI GREGORY J MORTON · 1986 to 2026
$30.4M
UW Center for Translational Muscle Research (Overall Application)P30AR074990 · NIAMS · UNIVERSITY OF WASHINGTON · PI Jennifer Michelle Davis, DANIEL RAFTERY · 2019 to 2026
$7.5M
Quantitative Analysis of Labile Metabolites in Biological SamplesR01GM138465 · NIGMS · UNIVERSITY OF WASHINGTON · PI GOWDA, G. A. NAGANA, RAFTERY, DANIEL · 2021 to 2024
$1.5M
NIAMS NIH HHS P30 AR074990NIDDK NIH HHS P30 DK035816NIDDK NIH HHS P30DK035816NIGMS NIH HHS R01 GM138465NIGMS NIH HHS R01GM138465
6 · The paper itself

Abstract

Investigation of mitochondrial metabolism is gaining increased interest owing to the growing recognition of the role of mitochondria in health and numerous diseases. Studies of isolated mitochondria promise novel insights into the metabolism devoid of confounding effects from other cellular organelles such as cytoplasm. This study describes the isolation of mitochondria from mouse skeletal myoblast cells (C2C12) and the investigation of live mitochondrial metabolism in real-time using isotope tracer-based NMR spectroscopy. [3-

Indexed as

MitochondriaPyruvic AcidAnimalsLactic AcidMagnetic Resonance SpectroscopyMiceLactic AcidPyruvic Acid[2-13C1]acetyl coenzyme A[3-13C1]lactate[3-13C1]pyruvatelive mitochondriametabolismNMR, 1H, 13C

Identifiers

PMID36882950
PMCPMC10483017

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.