ArticleScientific reports2023
Challenges in, and recommendations for, hyperspectral imaging in ex vivo malignant glioma biopsy measurements.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 21 citations in OpenAlex.
- Medical hyperspectral imaging: an updated review of technology advancements and biomedical applications.Journal of biomedical optics · 2026Pooled it
- Hyperspectral imaging for tumor resection guidance in surgery: a systematic review of preclinical and clinical studies.Journal of biomedical optics · 2025Pooled it
- Article
- Quantification of dual-state 5-ALA-induced PpIX fluorescence: methodology and validation in tissue-mimicking phantoms.Physics in medicine and biology · 2026Article
- Analysis of porphyrins from serum and the benefit of inert biphenyl columns for liquid chromatography separation.PloS one · 2026Article
- Integrating mass spectrometry and hyperspectral imaging for protoporphyrin IX detection in malignant glioma tissue.Scientific reports · 2025Article
- Review
- Probe-Based Fluorescence Spectroscopy for In Situ Brain Tumor Measurements During Resection and Needle Biopsies.Biomedicines · 2025Article
- Hyperspectral imaging in neurosurgery: a review of systems, computational methods, and clinical applications.Journal of biomedical optics · 2025Review
- Fluorescence-Guided Surgery Using 5-Aminolevulinic Acid/Protoporphyrin IX in Brain Metastases.Neurosurgery practice · 2024Review
- Spectral library and method for sparse unmixing of hyperspectral images in fluorescence guided resection of brain tumors.Biomedical optics express · 2024Article
- Towards machine learning-based quantitative hyperspectral image guidance for brain tumor resection.Communications medicine · 2024Article
- Machine and Deep Learning in Hyperspectral Fluorescence-Guided Brain Tumor Surgery.Advances in experimental medicine and biology · 2024Article
- Fluorescence guidance in skull base surgery: Applications and limitations - A systematic review.Brain & spine · 2024Review
- 5-ALA induced PpIX fluorescence spectroscopy in neurosurgery: a review.Frontiers in neuroscience · 2024Review
- A Wearable Fluorescence Imaging Device for Intraoperative Identification of Human Brain Tumors.IEEE journal of translational engineering in health and medicine · 2024Article
- Lightfield hyperspectral imaging in neuro-oncology surgery: an IDEAL 0 and 1 study.Frontiers in neuroscience · 2023Article
- Unraveling the blue shift in porphyrin fluorescence in glioma: The 620 nm peak and its potential significance in tumor biology.Frontiers in neuroscience · 2023Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The visualization of protoporphyrin IX (PPIX) fluorescence with the help of surgical microscopes during 5-aminolevulinic acid-mediated fluorescence-guided resection (FGR) of gliomas is still limited at the tumor margins. Hyperspectral imaging (HI) detects PPIX more sensitively but is not yet ready for intraoperative use. We illustrate the current status with three experiments and summarize our own experience using HI: (1) assessment of HI analysis algorithm using pig brain tissue, (2) a partially retrospective evaluation of our experience from HI projects, and (3) device comparison of surgical microscopy and HI. In (1), we address the problem that current algorithms for evaluating HI data are based on calibration with liquid phantoms, which have limitations. Their pH is low compared to glioma tissue; they provide only one PPIX photo state and only PPIX as fluorophore. Testing the HI algorithm with brain homogenates, we found proper correction for optical properties but not pH. Considerably more PPIX was measured at pH 9 than at pH 5. In (2), we indicate pitfalls and guide HI application. In (3), we found HI superior to the microscope for biopsy diagnosis (AUC = 0.845 ± 0.024 (cut-off 0.75 µg PPIX/ml) vs. 0.710 ± 0.035). HI thus offers potential for improved FGR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.