Evidence map›Paper›PMID 36881851›Full record

ArticleGenome biology and evolution2023

Tapping Culture Collections for Fungal Endophytes: First Genome Assemblies for Three Genera and Five Species in the Ascomycota.

Rowena Hill, Quentin Levicky, Frances Pitsillides, Amy Junnonen, Elena Arrigoni, J Miguel Bonnin, Anthony Kermode, Sahr Mian, Ilia J Leitch, Alan G Buddie and 2 more

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Article in Genome biology and evolution, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Rowena HillRoyal Botanic Gardens Kew, Richmond, UK.ORCID 0000-0002-1046-5528
Quentin LevickyDepartment of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, UK.
Frances PitsillidesRoyal Botanic Gardens Kew, Richmond, UK.
Amy JunnonenRoyal Botanic Gardens Kew, Richmond, UK.
Elena ArrigoniRoyal Botanic Gardens Kew, Richmond, UK.ORCID 0000-0002-4324-5521
J Miguel BonninCABI, Bakeham Lane, Egham, UK.ORCID 0000-0002-5095-6719
Anthony KermodeCABI, Bakeham Lane, Egham, UK.ORCID 0000-0002-2064-2081
Sahr MianRoyal Botanic Gardens Kew, Richmond, UK.ORCID 0000-0001-7828-0629
Ilia J LeitchRoyal Botanic Gardens Kew, Richmond, UK.ORCID 0000-0002-3837-8186
Alan G BuddieCABI, Bakeham Lane, Egham, UK.
Richard J A BuggsRoyal Botanic Gardens Kew, Richmond, UK.ORCID 0000-0003-4495-3738
Ester GayaRoyal Botanic Gardens Kew, Richmond, UK.ORCID 0000-0001-6404-4297

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Ascomycota form the largest phylum in the fungal kingdom and show a wide diversity of lifestyles, some involving associations with plants. Genomic data are available for many ascomycetes that are pathogenic to plants, but endophytes, which are asymptomatic inhabitants of plants, are relatively understudied. Here, using short- and long-read technologies, we have sequenced and assembled genomes for 15 endophytic ascomycete strains from CABI's culture collections. We used phylogenetic analysis to refine the classification of taxa, which revealed that 7 of our 15 genome assemblies are the first for the genus and/or species. We also demonstrated that cytometric genome size estimates can act as a valuable metric for assessing assembly "completeness", which can easily be overestimated when using BUSCOs alone and has broader implications for genome assembly initiatives. In producing these new genome resources, we emphasise the value of mining existing culture collections to produce data that can help to address major research questions relating to plant-fungal interactions.

Indexed as

AscomycotaEndophytesGenomicsPhylogenyAscomycotaculture collectionscytometric completenessfungal endophytes

Identifiers

PMID36881851
PMCPMC10027605

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Textfull text, public
LicenceCC BY
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measurements read8
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.