ArticleNucleic acids research2023
The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG.
Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 32 citations in OpenAlex.
- Nucleases and Their Inhibitors: Exploring Biological Roles, Industrial Applications, and Challenges in Heterologous Expression.Biotechnology journal · 2026Review
- Article
- Binding of Bacillus subtilis dynamin-like protein DynA to the bacterial membrane is essential for effective phage defense.The FEBS journal · 2026Article
- Supercoiled DNA recognition and cleavage control in topoisomerase VI.Nature communications · 2026Article
- DNA Bioconjugation with Polymer Beads Using SNAP-tag® Technology.Methods in molecular biology (Clifton, N.J.) · 2026Article
- SMC-like Wadjet system prevents plasmid transfer into Clostridium cellulovorans.Applied microbiology and biotechnology · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Early onset of septal FtsK localization allows for efficient DNA segregation in SMC-deletedmBio · 2025Article
- Nucleic acid recognition during prokaryotic immunity.Molecular cell · 2025Review
- Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex.Molecular cell · 2025Article
- Article
- MksB is a novel mycobacterial condensin that orchestrates spatiotemporal positioning of replication machinery.Scientific reports · 2024Article
- Defence systems and horizontal gene transfer in bacteria.Environmental microbiology · 2024Article
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Viral proteins activate PARIS-mediated tRNA degradation and viral tRNAs rescue infection.bioRxiv : the preprint server for biology · 2024Article
- SMC-based immunity against extrachromosomal DNA elements.Biochemical Society transactions · 2023Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells are continuously facing the risk of taking up foreign DNA that can compromise genomic integrity. Therefore, bacteria are in a constant arms race with mobile genetic elements such as phages, transposons and plasmids. They have developed several active strategies against invading DNA molecules that can be seen as a bacterial 'innate immune system'. Here, we investigated the molecular arrangement of the Corynebacterium glutamicum MksBEFG complex, which is homologous to the MukBEF condensin system. We show here that MksG is a nuclease that degrades plasmid DNA. The crystal structure of MksG revealed a dimeric assembly through its C-terminal domain that is homologous to the TOPRIM domain of the topoisomerase II family of enzymes and contains the corresponding ion binding site essential for DNA cleavage in topoisomerases. The MksBEF subunits exhibit an ATPase cycle in vitro and we reason that this reaction cycle, in combination with the nuclease activity provided by MksG, allows for processive degradation of invading plasmids. Super-resolution localization microscopy revealed that the Mks system is spatially regulated via the polar scaffold protein DivIVA. Introduction of plasmids results in an increase in DNA bound MksG, indicating an activation of the system in vivo.
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